CAJ | 학술논문

从山东省多地采集的花生茎腐病株上分离到15个茎腐病菌株，选取6个典型菌株，对病原菌进行形态特征、致病性、生物学特性研究。核糖体DNA-ITS序列分析表明，6个菌株的核糖体DNA-ITS序列同源性高达99％。根据病原菌的形态特征、核糖体DNA-ITS序列同源性比较，确认供试6个菌株为同一种病原菌，与Gen-Bank中可可毛色二孢属（Lasiodiplodia）（Diplodia的同物异名）的同源性高达99％以上。根据6个菌株的形态特征、致病性测定结果及生物学特性，并结合核糖体DNA-ITS序列分析，将山东花生茎腐病的病原鉴定为棉色二孢菌（Diplodia gossypina）。
종산동성다지채집적화생경부병주상분리도15개경부병균주，선취6개전형균주，대병원균진행형태특정、치병성、생물학특성연구。핵당체DNA-ITS서렬분석표명，6개균주적핵당체DNA-ITS서렬동원성고체99％。근거병원균적형태특정、핵당체DNA-ITS서렬동원성비교，학인공시6개균주위동일충병원균，여Gen-Bank중가가모색이포속（Lasiodiplodia）（Diplodia적동물이명）적동원성고체99％이상。근거6개균주적형태특정、치병성측정결과급생물학특성，병결합핵당체DNA-ITS서렬분석，장산동화생경부병적병원감정위면색이포균（Diplodia gossypina）。
Six representative strains were selected from peanut stem rot plants collected from Shandong Prov-ince.Morphological characteristics,pathogenicity and biological characteristics of 6 representative strains were studied.Ribosomal DNA-ITS sequence analysis showed that the sequence homology of these 6 strains was up to 99%.The tested 6 isolates were confirmed as one species.The homology of DNA-ITS sequences of the 6 strains was up to more than 99% compared with Lasiodiplodia spp (synonyms Diplodia)in GenBank.According to mor-phological characteristics,pathogenicity test and biological characteristics of the 6 tested strains,and combined with the ribosomal rDNA-ITS sequence analysis,the pathogen of peanut stem rot from Shandong Province was identi-fied as Diplodia gossypina.