CAJ | 학술논문

目的：检测人肿瘤坏死因子配体超家族成员13（ TNFSF13）在乳腺癌组织中的表达，并评估其作为乳腺癌患者潜在的预后生物标志物的可能性。方法采用Western blot和qRT-PCR两种方法检测TNFSF13在乳腺癌组织中的表达情况。结果 TNFSF13在乳腺癌组织中高表达，并且TNFSF13 mRNA的高表达与肿瘤分化程度和pN分期密切相关（P＝0．003，P＝0．022）。此外，Kaplan-Meier分析显示TNFSF13高表达的患者，生存时间显著较短（P＝0．04，log-rank检验）。 Cox风险比例模型多因素分析结果表明，TNFSF13的高表达是乳腺癌患者一个独立的预后因素（P＝0．03）。结论 TNFSF13有望成为乳腺癌患者预后判断及强化治疗的一个重要的新靶标。
목적：검측인종류배사인자배체초가족성원13（ TNFSF13）재유선암조직중적표체，병평고기작위유선암환자잠재적예후생물표지물적가능성。방법채용Western blot화qRT-PCR량충방법검측TNFSF13재유선암조직중적표체정황。결과 TNFSF13재유선암조직중고표체，병차TNFSF13 mRNA적고표체여종류분화정도화pN분기밀절상관（P＝0．003，P＝0．022）。차외，Kaplan-Meier분석현시TNFSF13고표체적환자，생존시간현저교단（P＝0．04，log-rank검험）。 Cox풍험비례모형다인소분석결과표명，TNFSF13적고표체시유선암환자일개독립적예후인소（P＝0．03）。결론 TNFSF13유망성위유선암환자예후판단급강화치료적일개중요적신파표。
Objective To detect the TNFSF13 expression and to evaluate its possible use as a potential prognostic marker in breast cancer .Methods We analyzed TNFSF13 expression by Western blot and qRT -PCR in 93 breast cancer patients .Results Our results showed that TNFSF 13 was overexpressed in breast cancer tis-sue.High TNFSF13 mRNA expression was closely correlated with tumor differentiation , pN classification ( P=0.003,0.022).In addition,patients with high TNFSF13 expression had a significantly shorter survival analyzed by Kaplan-Meier(P=0.04,log-rank test).Cox proportional hazards regression multivariable analysis revealed that high expression of TNFSF13 was identified as an independent prognostic factor (P=0.03).Conclusion TNFSF13 expression might be a novel target for prognosis and intensive therapy in breast cancer patients .