CAJ | 학술논문

目的：探讨信号转导子与转录激活子3（ STAT3）基因在肺腺癌细胞上皮-间质转化（ Epi-thelial-mesenchymal transition ，EMT）过程中的作用。方法应用STAT3-siRNA转染人肺腺癌A549细胞，下调STAT3基因表达。转染后观察细胞形态学变化，通过Western blot检测STAT3和Twist 蛋白的表达变化。同时应用Transwell法和划痕实验检测细胞的侵袭和迁移能力。并应用免疫组化法验证STAT3和Twist蛋白在肺腺癌组织中表达的相关性。结果 STAT 3-siRNA转染人肺腺癌A549细胞后，STAT3基因表达下降，同时降低Twist的表达，并且降低肺癌细胞的增殖、侵袭和迁移能力，在肺腺癌组织中我们也观察到随着分化程度升高，两个蛋白在肺癌组织中的的表达均逐渐降低，并呈正相关。结论 STAT3基因通过调节 Twist的表达，参与EMT过程，从而在肺癌进展中发挥重要作用。
목적：탐토신호전도자여전록격활자3（ STAT3）기인재폐선암세포상피-간질전화（ Epi-thelial-mesenchymal transition ，EMT）과정중적작용。방법응용STAT3-siRNA전염인폐선암A549세포，하조STAT3기인표체。전염후관찰세포형태학변화，통과Western blot검측STAT3화Twist 단백적표체변화。동시응용Transwell법화화흔실험검측세포적침습화천이능력。병응용면역조화법험증STAT3화Twist단백재폐선암조직중표체적상관성。결과 STAT 3-siRNA전염인폐선암A549세포후，STAT3기인표체하강，동시강저Twist적표체，병차강저폐암세포적증식、침습화천이능력，재폐선암조직중아문야관찰도수착분화정도승고，량개단백재폐암조직중적적표체균축점강저，병정정상관。결론 STAT3기인통과조절 Twist적표체，삼여EMT과정，종이재폐암진전중발휘중요작용。
Objective To study effects of signal transducer and activator of transcription 3(STAT3)gene on lung adenocarcinoma cells′EMT.Methods We observed morphological changes of the cells and detected the expression of STAT 3 and Twist by western blot after STAT3-siRNA plasmid was transfected into A549 cells. Meanwhile ,we observed invasion and migration ability of A 549 cells using transwell method and wound healing assay .Eventually ,we verified the correlation between STAT 3 and Twist expression in lung adenocarcinoma tissues by immunohistochemical method .Results si-STAT3 significantly down-regulated the expression of STAT 3 in A549 cells,and pronouncedly inhibited migration and invasion of A 549 cells in vitro.Meanwhile,we also found decreased expression of Twist .The expression of STAT3 was positively correlated with the expression of Twist in lung adenocarcinoma tissue specimens and the expression of two genes was reversely correlated with tissue differ -entiation degree .Conclusion The STAT3 participated in the process of EMT by regulating twist expression , which played a vital role in progression of lung adenocarcinoma .