CAJ | 학술논문

目的：探讨miR-124对紫杉醇诱导的乳腺癌细胞株MCF-7生长抑制作用的影响。方法应用MTT法检测紫杉醇对MCF-7的生长抑制作用，流式细胞仪检测紫杉醇对细胞周期的影响，应用实时荧光定量PCR（ qRT-PCR）技术检测紫杉醇处理MCF-7细胞后，miR-124表达水平的变化，进一步转染miR-124抑制剂至MCF-7细胞中，用MTT法检测细胞生长抑制情况。结果 MTT法检测结果显示紫杉醇能显著抑制MCF-7细胞生长。流式细胞仪检测结果显示紫杉醇能明显将细胞阻滞于G2期。 qRT-PCR检测结果显示紫杉醇能诱导miR-124表达水平升高，并呈现剂量依赖关系。进一步研究发现，抑制细胞miR-124的表达后，紫杉醇对MCF-7的增殖抑制作用受到显著影响。结论紫杉醇能显著诱导MCF-7上调表达miR-124，并且这种上调表达是剂量依赖性的。而miR-124抑制剂能明显降低紫杉醇对MCF-7细胞的生长抑制作用。表明miR-124可能在细胞对紫杉醇的化疗耐药中起着重要作用，也为将来解决紫杉醇临床耐药提供了新思路。
목적：탐토miR-124대자삼순유도적유선암세포주MCF-7생장억제작용적영향。방법응용MTT법검측자삼순대MCF-7적생장억제작용，류식세포의검측자삼순대세포주기적영향，응용실시형광정량PCR（ qRT-PCR）기술검측자삼순처리MCF-7세포후，miR-124표체수평적변화，진일보전염miR-124억제제지MCF-7세포중，용MTT법검측세포생장억제정황。결과 MTT법검측결과현시자삼순능현저억제MCF-7세포생장。류식세포의검측결과현시자삼순능명현장세포조체우G2기。 qRT-PCR검측결과현시자삼순능유도miR-124표체수평승고，병정현제량의뢰관계。진일보연구발현，억제세포miR-124적표체후，자삼순대MCF-7적증식억제작용수도현저영향。결론자삼순능현저유도MCF-7상조표체miR-124，병차저충상조표체시제량의뢰성적。이miR-124억제제능명현강저자삼순대MCF-7세포적생장억제작용。표명miR-124가능재세포대자삼순적화료내약중기착중요작용，야위장래해결자삼순림상내약제공료신사로。
Objective Our study aims to investigate the inhibitive effects of miR -124 on the growth of breast cancer cell MCF -7 induced by paclitaxel .Methods MTT was used to detect the growth inhibition of MCF-7 induced by paclitaxel .Flow cytometry was used to detect the effect of paclitaxel on cell cycle .Real-time quantitative PCR(qRT-PCR) was used to detect the expressive level of miR -124,while MCF -7 cells were treated with paclitaxel .MiR-124 inhibitor was transfected into MCF -7 breast cancer cells ,and growth in-hibition was detected by MTT .Results The results showed that paclitaxel could significantly inhibit the growth of breast cancer cell line MCF -7 by blocking the G2 phase.The results from qRT-PCR showed that the relative expression of miR-124 was increased when the dosage of paclitaxel was increased .When the expression of miR-124 was inhibited ,the cell growth inhibition caused by paclitaxel was also prominently decreased .Conclusion The higher expression of miR -124 in MCF-7 induced by paclitaxel was dose dependent .And miR-124 in-hibitor can significantly influence the cell growth inhibition caused by paclitaxel .These results indicat that miR -124 plays an important role in paclitaxel -induced chemotherapy drug resistance ,and provides a new direction to solve the problem .