内蒙古农业科技
內矇古農業科技
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INNER MONGOLIA AGRICULTURAL SCIENCE AND TECHNOLOGY
2014年
4期
25-27,49
,共4页
丛枝菌根真菌%不同盐度样地%羊草%侵染状况
叢枝菌根真菌%不同鹽度樣地%羊草%侵染狀況
총지균근진균%불동염도양지%양초%침염상황
Arbuscular mycorrhizal fungi%Different salinities%Leymus chinense%Colonization
对松嫩盐碱草地的羊草(Leymus chinense)和丛枝菌根(Arbuscular mycorrhizal, AM)真菌之间的关系进行了调查,分别采集了轻、中、重三个盐度样地的羊草根系, Trypan-blue染色和制片后镜检丛枝、泡囊、菌丝等形态结构类型,计算出侵染率(%)、侵染强度(%)、从枝丰度(%)和泡囊丰度(%)。结果表明:所有盐度样地均能侵染成典型的AM真菌结构,中盐度样地的AM真菌侵染率、泡囊丰度和丛枝丰度均为三个盐度样地的最大值,达到了100%、31.78%和76.52%,重盐度样地的侵染率、侵染强度、泡囊丰度和丛枝丰度均为三个盐度样地的最小值,仅有90%、27.15%、16.90%和57.74%。同一盐度样地之间AM真菌的侵染状况也有显著性差异(P<0.05);轻盐度和中盐度的菌根类型是A-型,重盐度样地的菌根类型是P-型。
對鬆嫩鹽堿草地的羊草(Leymus chinense)和叢枝菌根(Arbuscular mycorrhizal, AM)真菌之間的關繫進行瞭調查,分彆採集瞭輕、中、重三箇鹽度樣地的羊草根繫, Trypan-blue染色和製片後鏡檢叢枝、泡囊、菌絲等形態結構類型,計算齣侵染率(%)、侵染彊度(%)、從枝豐度(%)和泡囊豐度(%)。結果錶明:所有鹽度樣地均能侵染成典型的AM真菌結構,中鹽度樣地的AM真菌侵染率、泡囊豐度和叢枝豐度均為三箇鹽度樣地的最大值,達到瞭100%、31.78%和76.52%,重鹽度樣地的侵染率、侵染彊度、泡囊豐度和叢枝豐度均為三箇鹽度樣地的最小值,僅有90%、27.15%、16.90%和57.74%。同一鹽度樣地之間AM真菌的侵染狀況也有顯著性差異(P<0.05);輕鹽度和中鹽度的菌根類型是A-型,重鹽度樣地的菌根類型是P-型。
대송눈염감초지적양초(Leymus chinense)화총지균근(Arbuscular mycorrhizal, AM)진균지간적관계진행료조사,분별채집료경、중、중삼개염도양지적양초근계, Trypan-blue염색화제편후경검총지、포낭、균사등형태결구류형,계산출침염솔(%)、침염강도(%)、종지봉도(%)화포낭봉도(%)。결과표명:소유염도양지균능침염성전형적AM진균결구,중염도양지적AM진균침염솔、포낭봉도화총지봉도균위삼개염도양지적최대치,체도료100%、31.78%화76.52%,중염도양지적침염솔、침염강도、포낭봉도화총지봉도균위삼개염도양지적최소치,부유90%、27.15%、16.90%화57.74%。동일염도양지지간AM진균적침염상황야유현저성차이(P<0.05);경염도화중염도적균근류형시A-형,중염도양지적균근류형시P-형。
The relationship between Leymus chinense and AM (arbuscular mycorrhizal) fungi in different salinities of Songnen alkaline grassland was investigated in this study, the roots of Leymus chinense from low salinity, middle salinity and high salinity were gathered respectively. Using microscopy techniques, arbuscular, vesicles,and other forms of mycorrhizal structure type were observed after Trypan-blue staining and producer. Results showed that AM fungi colonized all Leymus chinense in different salinitie, among which middle salinity got the highest colonization rate, infection intensity, vesicular abundance and arbuscular abundance of 100%,31.78% and 76.25%,while high salinity got the lowest colonization rate, infection intensity, vesicular abundance and arbuscular abundance of only 90%,27.15%,16.90%and 57.74%.There were also significant differences on the colonization of Leymus chinense arbuscular mycorrhizal in the same salinity (P<0.05). The AM type of Leymus chinense was A-type in low salinity and middle salinity, while the AM type of high salinity was P-type.