CAJ | 학술논문

目的：观察妊娠期糖尿病（ GDM）产妇胎盘组织内质网应激（ ERS）发生情况并探讨其对胎盘组织形态学的影响。方法 GDM产妇（GDM组）和健康产妇（对照组）各30例，分娩时收集胎盘组织，Western blot 法检测胎盘组织中的磷酸化蛋白激酶R样内质网激酶（ p-PERK ）、CCAAT/增强子结合蛋白同源蛋白（ CHOP ）。 HE染色对胎盘组织行病理学观察。结果 GDM组p-PERK、CHOP相对表达量均高于对照组（ P均＜0．05）。病理检查显示， GDM组胎盘组织出现绒毛成熟不良、干绒毛小动脉管壁增厚及管腔狭窄、合体细胞结节增多、绒毛间质内毛细血管过度充盈、细胞滋养叶细胞及血管合体膜形成增加的例数高于对照组（P均＜0．05）。 GDM组胎盘组织中p-PERK、CHOP表达与胎盘组织异常形态学改变呈正相关关系（r分别为0．359、0．366，P均＜0．05）。结论 GDM产妇胎盘组织呈ERS状态，其与产妇胎盘组织异常形态学改变有关。
목적：관찰임신기당뇨병（ GDM）산부태반조직내질망응격（ ERS）발생정황병탐토기대태반조직형태학적영향。방법 GDM산부（GDM조）화건강산부（대조조）각30례，분면시수집태반조직，Western blot 법검측태반조직중적린산화단백격매R양내질망격매（ p-PERK ）、CCAAT/증강자결합단백동원단백（ CHOP ）。 HE염색대태반조직행병이학관찰。결과 GDM조p-PERK、CHOP상대표체량균고우대조조（ P균＜0．05）。병리검사현시， GDM조태반조직출현융모성숙불량、간융모소동맥관벽증후급관강협착、합체세포결절증다、융모간질내모세혈관과도충영、세포자양협세포급혈관합체막형성증가적례수고우대조조（P균＜0．05）。 GDM조태반조직중p-PERK、CHOP표체여태반조직이상형태학개변정정상관관계（r분별위0．359、0．366，P균＜0．05）。결론 GDM산부태반조직정ERS상태，기여산부태반조직이상형태학개변유관。
Objective To investigate the ralationship between endoplasmic reticulum stress ( ERS ) and damage of histomorphology of placentas in gestational diabetes mellitus (GDM).Methods Thirty women with GDM(GDM group) and 30 normal pregnan women ( control group ) were included in this study .Placentas of all women in two groups were col-lected during the delivery .The expression of phosphorylated protein kinase R-like ER kinase ( p-PERK) and CCAAT/en-hancer-binding protein homologous protein ( CHOP ) in two groups were deteced by western blot method .The features of placentas pathologicchanges were observed by using microscopy .Results The expression phosphorylated PERK and CHOP protein were significantly increased in GDM group as compared with the control group (all P<0.05).Placentas obtained from GDM group had immature villi , thickening of trunk villus small artery wall , narrowing of lumen , increasing number of syncytial sprouts , the interstitial blood capillary expanding and engorge , proliferating cytotrophoblasts and thickening of vas-culo-syncytial membrane under light microscopy .Placentas from control group did not show the pathologic changes as men-tioned above.Bivariate analysis showed that lymphatic p-PERK and CHOP expression were positively correlated with the damage of histomorphology of placentas in GDM (r=0.359, 0.366, respectively,all P<0.05).Conclusion ERS was overexpressed in placental tissue of DGM and may be positive related with the damage of histomorphology of the placentas .