国际药学研究杂志
國際藥學研究雜誌
국제약학연구잡지
INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH
2014年
4期
478-482,489
,共6页
王林%张刘珍%丛悦%善亚君%柳晓兰%崔宇%王丽梅%熊国林%邢爽%从玉文%余祖胤
王林%張劉珍%叢悅%善亞君%柳曉蘭%崔宇%王麗梅%熊國林%邢爽%從玉文%餘祖胤
왕림%장류진%총열%선아군%류효란%최우%왕려매%웅국림%형상%종옥문%여조윤
CBLB502%急性放射病%造血损伤%造血干细胞
CBLB502%急性放射病%造血損傷%造血榦細胞
CBLB502%급성방사병%조혈손상%조혈간세포
CBLB502%acute radiation disease%haemopoiesis injury%hemopoietic stem cell
目的：研究CBLB502对辐射造血损伤的防护作用。方法C57BL/6J小鼠随机分为正常对照、60Co γ射线6.5Gy一次性全身照射对照和CBLB502照前30 min 0.2mg/kg皮下给药预防组，照前1 d和照后30 d定期检测外周血细胞数，照射后2和24 h取骨髓检测有核细胞数和集落形成数，竞争性移植方法观察骨髓造血细胞植入能力，流式细胞仪测定外周血中各系细胞比例。结果CBLB502能显著减轻60Co γ射线6.5 Gy照射小鼠的外周血中白细胞、红细胞及血小板数的急剧降低并加速其恢复，照后2和24 h CBLB502预防给药组小鼠骨髓各系造血祖细胞集落形成数均显著高于照射对照组（P<0.01），且预防给药可明显提高受照射小鼠骨髓造血细胞的植入能力。结论 CBLB502具有明显的辐射造血损伤保护作用。
目的：研究CBLB502對輻射造血損傷的防護作用。方法C57BL/6J小鼠隨機分為正常對照、60Co γ射線6.5Gy一次性全身照射對照和CBLB502照前30 min 0.2mg/kg皮下給藥預防組，照前1 d和照後30 d定期檢測外週血細胞數，照射後2和24 h取骨髓檢測有覈細胞數和集落形成數，競爭性移植方法觀察骨髓造血細胞植入能力，流式細胞儀測定外週血中各繫細胞比例。結果CBLB502能顯著減輕60Co γ射線6.5 Gy照射小鼠的外週血中白細胞、紅細胞及血小闆數的急劇降低併加速其恢複，照後2和24 h CBLB502預防給藥組小鼠骨髓各繫造血祖細胞集落形成數均顯著高于照射對照組（P<0.01），且預防給藥可明顯提高受照射小鼠骨髓造血細胞的植入能力。結論 CBLB502具有明顯的輻射造血損傷保護作用。
목적：연구CBLB502대복사조혈손상적방호작용。방법C57BL/6J소서수궤분위정상대조、60Co γ사선6.5Gy일차성전신조사대조화CBLB502조전30 min 0.2mg/kg피하급약예방조，조전1 d화조후30 d정기검측외주혈세포수，조사후2화24 h취골수검측유핵세포수화집락형성수，경쟁성이식방법관찰골수조혈세포식입능력，류식세포의측정외주혈중각계세포비례。결과CBLB502능현저감경60Co γ사선6.5 Gy조사소서적외주혈중백세포、홍세포급혈소판수적급극강저병가속기회복，조후2화24 h CBLB502예방급약조소서골수각계조혈조세포집락형성수균현저고우조사대조조（P<0.01），차예방급약가명현제고수조사소서골수조혈세포적식입능력。결론 CBLB502구유명현적복사조혈손상보호작용。
Objective To study the radioprotective potential of CBLB502 protein against hemopoiesis injury by irradiat-ion. Methods C57BL/6J mice were assigned to normal irradiation, and CBLB502 0.2mg/kg 30min pre-irradiation group at random before 6.5 Gy 60Co-γray total body irradiation (TBI). The peripheral blood was obtained to assay hemogram pre and post-irradiation. The bone marrow nucleated cell counts were evaluated in mice at 2 h and 24 h after irradiation. Colony-forming unit (CFU) assay was used to analyze the alteration of hemopoietic progenitor cells in bone marrow. Competitive repopulation assay was conducted to observe the implantation ability of bone marrow cells. The percentage of each lineage hemopoietic cells was measured by flow cytometry analysis. Results CBLB502 significantly alleviated the sharp decrease of peripheral blood counts including leukocyte (WBC), erythrocyte (RBC), and platelet, and accelerated recovery. Counts of various hematopoietic progenitor cell colonies of mouse bone marrow in CBLB502 group were significant higher than those of irradiation group (P<0.01). CBLB502 significantly improved the implantation ability of bone marrow cells. Conclusion CBLB502 showed obvious radioprotective effects on haemopoiesis injury by irradiation.
