CAJ | 학술논문

目的：比较采用不同的消退方法对吗啡诱导条件性位置偏爱（CPP）的消退及随后小剂量药物诱发点燃的影响。方法小鼠采用交替隔日皮下注射吗啡（10 mg/kg）或等容量生理盐水后在伴药箱或非伴药箱训练共8 d，即接受吗啡和生理盐水训练共4个轮次，使小鼠形成CPP。采用不同的消退方法使小鼠CPP熄灭。方案1：自然消退。小鼠吗啡CPP形成后不做任何处理，待21 d后，每隔7 d进行1次测试，观察其消退程度。方案2：测试消退。小鼠吗啡CPP形成第2天起，每日进行1次消退测试观察其消退程度；方案3：训练消退。小鼠吗啡CPP形成第2天起，用生理盐水代替吗啡，小鼠每日分别在伴药箱和非伴药箱完成一轮训练，每训练2轮测试1次，观察其消退程度。待各组完全消退后间隔2～7 d，皮下注射5 mg/kg吗啡对小鼠CPP进行重建测试。结果吗啡处理诱导小鼠出现显著的CPP效应，自然消退组在CPP形成后第28天仍保持CPP行为，第35天CPP效应消失。消退测试6次，训练熄灭4轮均使得CPP效应消退。以上3种方案消退的小鼠均可被5 mg/kg的吗啡点燃。结论吗啡诱导的小鼠CPP模型可维持28 d以上（自然消退），测试及训练消退的方法均可加速其消退，且5 mg/kg吗啡可诱导各组CPP效应的重建。
목적：비교채용불동적소퇴방법대마배유도조건성위치편애（CPP）적소퇴급수후소제량약물유발점연적영향。방법소서채용교체격일피하주사마배（10 mg/kg）혹등용량생리염수후재반약상혹비반약상훈련공8 d，즉접수마배화생리염수훈련공4개륜차，사소서형성CPP。채용불동적소퇴방법사소서CPP식멸。방안1：자연소퇴。소서마배CPP형성후불주임하처리，대21 d후，매격7 d진행1차측시，관찰기소퇴정도。방안2：측시소퇴。소서마배CPP형성제2천기，매일진행1차소퇴측시관찰기소퇴정도；방안3：훈련소퇴。소서마배CPP형성제2천기，용생리염수대체마배，소서매일분별재반약상화비반약상완성일륜훈련，매훈련2륜측시1차，관찰기소퇴정도。대각조완전소퇴후간격2～7 d，피하주사5 mg/kg마배대소서CPP진행중건측시。결과마배처리유도소서출현현저적CPP효응，자연소퇴조재CPP형성후제28천잉보지CPP행위，제35천CPP효응소실。소퇴측시6차，훈련식멸4륜균사득CPP효응소퇴。이상3충방안소퇴적소서균가피5 mg/kg적마배점연。결론마배유도적소서CPP모형가유지28 d이상（자연소퇴），측시급훈련소퇴적방법균가가속기소퇴，차5 mg/kg마배가유도각조CPP효응적중건。
Objective To compare the effect of extinction using different methods and the subsequent reinstatement of morphine-primed on morphine-induced conditioned place preference (CPP). Methods Mouse received morphine(10 mg/kg, sc) or saline (10 mg/kg, sc) injections alternately for 8 days to establish CPP. Different methods were used for extinction. Program 1, withdrawal group. Extinction sessions were conducted 21 days after the CPP test at 7 days interval; Program 2, extinction test group. Extinction sessions were carried out once a day from the second day after the CPP test; Program 3, extinction training group. Extinction sessions began one day after the CPP test. Mice were treated alternately two trials daily, in compartments where they received saline instead of morphine. A test for reinstatement was performed on the 2nd or 7th day after all groups completed extinction by 5 mg/kg morphine-primed. Results Morphine injections induce strong CPP. In withdrawal group, CPP extinguished on the 35th day and maintained for at least 28 days. Six trials extinction tests and 4 trials extinction training caused morphine induced CPP extinction. The three programs mentioned above were all primed by 5mg/kg morphine. Conclusion Morphine induced mouse CPP model can maintain at least 28 days, both extinction test and training can accelerate the extinction of CPP, and reinstatement by 5 mg/kg morphine.