国际药学研究杂志
國際藥學研究雜誌
국제약학연구잡지
INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH
2014年
4期
468-472
,共5页
李晶晶%王晓娟%刘妍如%杨建云%肖炳坤%黄荣清
李晶晶%王曉娟%劉妍如%楊建雲%肖炳坤%黃榮清
리정정%왕효연%류연여%양건운%초병곤%황영청
代谢组学%1H NMR%川楝子%血清
代謝組學%1H NMR%川楝子%血清
대사조학%1H NMR%천련자%혈청
metabonomics%1H NMR%Fructus Toosendan%serum
目的:基于1H NMR技术和主成分分析(principal component analysis,PCA)方法,观察给予川楝子乙酸乙酯提取物后小鼠血清代谢物组的变化,并对其毒性机制进行解释。方法给药组灌服川楝子乙酸乙酯提取物,采集血清进行1H NMR检测,利用主成分分析法对小鼠血清内源性小分子代谢产物进行分析处理。建立川楝子毒性模型并收集数据后,运用PCA载荷图对磁共振氢谱和相关代谢产物进行鉴定和分类。结果 PCA三维得分图中给药组与对照组无明显重叠部分,载荷图中显示最大变异代谢物,生物标志物变化明显,发现小鼠血清中参与能量代谢的葡萄糖和乳酸含量增加,甘氨酸等氨基酸水平明显上升,胆碱水平下降。结论结合小鼠体质量和血清生化指标,显示川楝子乙酸乙酯提取物具有肝肾毒性。
目的:基于1H NMR技術和主成分分析(principal component analysis,PCA)方法,觀察給予川楝子乙痠乙酯提取物後小鼠血清代謝物組的變化,併對其毒性機製進行解釋。方法給藥組灌服川楝子乙痠乙酯提取物,採集血清進行1H NMR檢測,利用主成分分析法對小鼠血清內源性小分子代謝產物進行分析處理。建立川楝子毒性模型併收集數據後,運用PCA載荷圖對磁共振氫譜和相關代謝產物進行鑒定和分類。結果 PCA三維得分圖中給藥組與對照組無明顯重疊部分,載荷圖中顯示最大變異代謝物,生物標誌物變化明顯,髮現小鼠血清中參與能量代謝的葡萄糖和乳痠含量增加,甘氨痠等氨基痠水平明顯上升,膽堿水平下降。結論結閤小鼠體質量和血清生化指標,顯示川楝子乙痠乙酯提取物具有肝腎毒性。
목적:기우1H NMR기술화주성분분석(principal component analysis,PCA)방법,관찰급여천련자을산을지제취물후소서혈청대사물조적변화,병대기독성궤제진행해석。방법급약조관복천련자을산을지제취물,채집혈청진행1H NMR검측,이용주성분분석법대소서혈청내원성소분자대사산물진행분석처리。건립천련자독성모형병수집수거후,운용PCA재하도대자공진경보화상관대사산물진행감정화분류。결과 PCA삼유득분도중급약조여대조조무명현중첩부분,재하도중현시최대변이대사물,생물표지물변화명현,발현소서혈청중삼여능량대사적포도당화유산함량증가,감안산등안기산수평명현상승,담감수평하강。결론결합소서체질량화혈청생화지표,현시천련자을산을지제취물구유간신독성。
Objective 1H NMR spectroscopy and principal component analysis(PCA) were used to investigate the mouse serum metabonomics and toxic mechanism after oral administration of Fructus Toosendan. Methods In this study, mice were orally administered with the ethyl acetate extract of Fructus Toosendan before collecting serum samples. Then 1H NMR was utilized to identify the metabolites with the PCA. After the experimental model of toxicity was established and the data were collected, NMR spectral regions and the metabolites were identified and ranked according to their weight aline with the loading plot. Results The scores plot for classification within groups was clear and showed a great difference from the control group. Combining the body weight and biochemical results, the elevated levels of glucose, lactate, taurine, arginine, glycine and decreased levels of betaine, creatine, amino and neurotransmitters were observed. Conclusion Our current study indicated that the ethyl acetate extract of Fructus Toosendan has liver and kidney toxicity.