CAJ | 학술논문

目的：阐明南酸枣中没食子酸衍生物类化学成分及其体外抗肿瘤、抗缺氧、抗菌活性。方法利用各种色谱技术分离化学成分，根据理化性质和光谱数据鉴定结构，利用MTT法测评抗肿瘤与抗缺氧活性，用纸片法测定抗菌活性。结果从南酸枣树皮中分离鉴定了没食子酸（1）、没食子酸乙酯（2）、1-O-没食子酰基-β-D-吡喃葡萄糖（3）、1，6-二-O-没食子酰基-β-D-吡喃葡萄糖（4）、1，4-二-O-没食子酰基-β-D-吡喃葡萄糖（5）、1，4，6-三-O-没食子酰基-β-D-吡喃葡萄糖（6）、1，3，4，6-四-O-没食子酰基-β-D-吡喃葡萄糖（7）等7个没食子酸衍生物类化合物。化合物1、2、4~6对K562细胞呈较强抗肿瘤活性，IC50分别为2.9、14.6、39.1、40.2和41.2μg /ml；化合物3和7活性较弱，在100μg /ml浓度下对K562细胞的抑制率分别为20.8%和30.1%。在对ECV304和PC12细胞的缺氧损伤保护实验中，1~7在无明显细胞毒作用的浓度下呈较强的抗缺氧活性。化合物5~7还对受试金黄色葡萄球菌显示一定的抗菌活性。结论化合物2~7为首次从南酸枣属植物分离得到。化合物1~7作为南酸枣中抗肿瘤、抗缺氧活性成分系首次报道，其抗缺氧活性也属首次报道。
목적：천명남산조중몰식자산연생물류화학성분급기체외항종류、항결양、항균활성。방법이용각충색보기술분리화학성분，근거이화성질화광보수거감정결구，이용MTT법측평항종류여항결양활성，용지편법측정항균활성。결과종남산조수피중분리감정료몰식자산（1）、몰식자산을지（2）、1-O-몰식자선기-β-D-필남포도당（3）、1，6-이-O-몰식자선기-β-D-필남포도당（4）、1，4-이-O-몰식자선기-β-D-필남포도당（5）、1，4，6-삼-O-몰식자선기-β-D-필남포도당（6）、1，3，4，6-사-O-몰식자선기-β-D-필남포도당（7）등7개몰식자산연생물류화합물。화합물1、2、4~6대K562세포정교강항종류활성，IC50분별위2.9、14.6、39.1、40.2화41.2μg /ml；화합물3화7활성교약，재100μg /ml농도하대K562세포적억제솔분별위20.8%화30.1%。재대ECV304화PC12세포적결양손상보호실험중，1~7재무명현세포독작용적농도하정교강적항결양활성。화합물5~7환대수시금황색포도구균현시일정적항균활성。결론화합물2~7위수차종남산조속식물분리득도。화합물1~7작위남산조중항종류、항결양활성성분계수차보도，기항결양활성야속수차보도。
Objective To investigate gallic acid-derived chemical constituents of Choerospondias axillaries (Roxb.) Burtt. et Hill., and evaluate their in vitro anti-tumor, anti-hypoxia and anti-bacteria activities. Methods The aimed chemical constituents were isolated by various chromatographic means, and their structures were identified by physicochemical and spectroscopic data. MTT method was employed to evaluate anti-tumor and anti-hypoxia activities. Antibacterial activities were tested by paper disc method. Results Seven compounds 1-7 were isolated from the stem barks of Choerospondias axillaries (Roxb.) Burtt. et Hill. and identified as gallic acid(1), gallic acid ethyl ether(2), 1-O-galloyl-β-D-glucose(3), 1,6-di-O-galloyl-β-D-glucose(4), 1,4-di-O-galloyl-β-D-glucose(5), 1,4,6-tri-O-galloyl-β-D-glucose(6), and 1,3,4,6-tetra-O-galloyl-β-D-glucose(7). Compounds 1, 2 and 4-6 significantly inhibited K562 cells with the IC50 values of 2.9, 14.6, 39.1, 40.2, 41.2 μg/ml, respectively, and 3 and 7 also showed a slight inhibition of the K562 cells with the inhibition rate of 20.8% and 30.1% at 100 μg/ml respectively. Compounds 1-7 showed protective effects on anoxia-induced injury in cultured ECV304 and PC12 cells at the concentrations showing no significant cytotoxicity, and 5-7 also showed an antibacterial effect on Staphylococcus aureus ATCC6538 to a certain extent. Conclusion Compounds 2-7 were isolated from the genus Choerospondias for the first time. It was the first time to report 1-7 as anti-tumor and anti-hypoxia constituents of Choerospondias axillaries, and the anti-hypoxia activity for 1-7 was also recorded for the first time in the present study.