国际妇产科学杂志
國際婦產科學雜誌
국제부산과학잡지
JOURNAL OF INTERNATIONAL OBSTETRICS AND GYNECOLOGY
2014年
4期
370-373
,共4页
宫颈肿瘤%癌%基因,肿瘤抑制%基因%NF-κB%免疫组织化学%乳腺癌转移抑制基因1
宮頸腫瘤%癌%基因,腫瘤抑製%基因%NF-κB%免疫組織化學%乳腺癌轉移抑製基因1
궁경종류%암%기인,종류억제%기인%NF-κB%면역조직화학%유선암전이억제기인1
Uterine cervical neoplasms%Carcinoma%Genes,tumor suppressor%Genes%NF-kappa B%Immunohistochemistry%Breast cancer metastasis suppressor 1
目的：探讨乳腺癌转移抑制基因1（BRMS1）、核因子相关κB结合蛋白50（NF-κB p50）在宫颈癌变过程中的表达及其临床意义。方法：采用免疫组化（Super Sensitive TM IHC）法分别检测慢性宫颈炎组织、CINⅠ组织、CINⅡ～Ⅲ组织和宫颈癌组织中BRMS1、NF-κB p50的表达，分析两者的表达水平与宫颈癌临床病理特征的关系。结果：①宫颈癌、CINⅡ～Ⅲ及CINⅠ组组织中BRMS1蛋白的表达低于慢性宫颈炎组，差异有统计学意义（均P＜0.05），且BRMS1蛋白在ⅡB～Ⅳ期、浸润深度＞1/2、有淋巴结转移者中表达降低甚至缺失（均P＜0.05）。②NF-κB p50蛋白在宫颈癌组、CINⅠ和CINⅡ～Ⅲ组中的阳性表达率高于慢性宫颈炎组（均P＜0.05），且在瘤体直径≥4 cm、低分化癌、浸润深度＞1/2、有淋巴结转移者中NF-κB p50蛋白表达升高，差异有统计学意义（均P＜0.05）。③宫颈癌中BRMS1与NF-κB p50蛋白表达水平呈负相关（rs=0.426，P＜0.001）。结论：宫颈癌中BRMS1蛋白与NF-κB蛋白的阳性表达呈负相关，BRMS1蛋白阳性表达逐渐下降、甚至缺失，而NF-κB蛋白阳性表达逐渐升高，与癌细胞的发展、浸润转移密切相关。
目的：探討乳腺癌轉移抑製基因1（BRMS1）、覈因子相關κB結閤蛋白50（NF-κB p50）在宮頸癌變過程中的錶達及其臨床意義。方法：採用免疫組化（Super Sensitive TM IHC）法分彆檢測慢性宮頸炎組織、CINⅠ組織、CINⅡ～Ⅲ組織和宮頸癌組織中BRMS1、NF-κB p50的錶達，分析兩者的錶達水平與宮頸癌臨床病理特徵的關繫。結果：①宮頸癌、CINⅡ～Ⅲ及CINⅠ組組織中BRMS1蛋白的錶達低于慢性宮頸炎組，差異有統計學意義（均P＜0.05），且BRMS1蛋白在ⅡB～Ⅳ期、浸潤深度＞1/2、有淋巴結轉移者中錶達降低甚至缺失（均P＜0.05）。②NF-κB p50蛋白在宮頸癌組、CINⅠ和CINⅡ～Ⅲ組中的暘性錶達率高于慢性宮頸炎組（均P＜0.05），且在瘤體直徑≥4 cm、低分化癌、浸潤深度＞1/2、有淋巴結轉移者中NF-κB p50蛋白錶達升高，差異有統計學意義（均P＜0.05）。③宮頸癌中BRMS1與NF-κB p50蛋白錶達水平呈負相關（rs=0.426，P＜0.001）。結論：宮頸癌中BRMS1蛋白與NF-κB蛋白的暘性錶達呈負相關，BRMS1蛋白暘性錶達逐漸下降、甚至缺失，而NF-κB蛋白暘性錶達逐漸升高，與癌細胞的髮展、浸潤轉移密切相關。
목적：탐토유선암전이억제기인1（BRMS1）、핵인자상관κB결합단백50（NF-κB p50）재궁경암변과정중적표체급기림상의의。방법：채용면역조화（Super Sensitive TM IHC）법분별검측만성궁경염조직、CINⅠ조직、CINⅡ～Ⅲ조직화궁경암조직중BRMS1、NF-κB p50적표체，분석량자적표체수평여궁경암림상병리특정적관계。결과：①궁경암、CINⅡ～Ⅲ급CINⅠ조조직중BRMS1단백적표체저우만성궁경염조，차이유통계학의의（균P＜0.05），차BRMS1단백재ⅡB～Ⅳ기、침윤심도＞1/2、유림파결전이자중표체강저심지결실（균P＜0.05）。②NF-κB p50단백재궁경암조、CINⅠ화CINⅡ～Ⅲ조중적양성표체솔고우만성궁경염조（균P＜0.05），차재류체직경≥4 cm、저분화암、침윤심도＞1/2、유림파결전이자중NF-κB p50단백표체승고，차이유통계학의의（균P＜0.05）。③궁경암중BRMS1여NF-κB p50단백표체수평정부상관（rs=0.426，P＜0.001）。결론：궁경암중BRMS1단백여NF-κB단백적양성표체정부상관，BRMS1단백양성표체축점하강、심지결실，이NF-κB단백양성표체축점승고，여암세포적발전、침윤전이밀절상관。
Objective:To study the protein expression and clinical significance of breast cancer metastasis suppressor l (BRMS1) and nuclear factor kappa B (NF-κB p50) in cervical carcinogenesis. Methods:The immunohistochemical method (Super Sensitive TM IHC)was used to determine expressions of BRMS1 and NF-κB p50 were in chronic cervicitis tissues,CINⅠ tissues,CINⅡ-Ⅲ tissues and cervical carcinoma tissues. The clinical significance was analyzed with histopalthological records. Results:①The positive rates of BRMS1 in cervical carcinoma ,CINⅠand CINⅡ-Ⅲtissues were much lower than that in chronic cervicitis tissues, the differences were significant (all P<0.05),moreover the positive expressions were much lower or absent in cervical cancer tissues withⅡB-Ⅳstage,depth of invasion>1/2 and with lymph node metastasis, the differences were significant (all P<0.05).②The positive rates of NF-κB p50 in cervical carcinoma,CINⅠand CINⅡ-Ⅲtissues were much higher than that in chronic cervicitis tissues(all P<0.05),moreover the positive expressions were much higher in cervical cancer tissues with tumor size≥4 cm,poor differentiation,depth of invasion>1/2 and with lymph node metastasis, the differences were significant (all P<0.05). ③The expression level of BRMS1 was negatively correlated with that of NF-κB p50 in cervical carcinoma (rs=0.426,P<0.001). Conclusions:It is closely related to cancer cell invasion and metastasis that the expression of BRMS1 decreased significantly,while the expression of NF-κB conversely increased. In addition,BRMS1 may inhibit NF-κB expression in invasion and metastasis of cervical cancer.
