CAJ | 학술논문

采用微波萃取法优化枸杞中类胡萝卜素的提取工艺，同时对枸杞鲜果冻干制品中类胡萝卜素的含量及清除DPPH的能力进行了测定。以β-胡萝卜素为对照品，采用正交实验方法研究了微波萃取温度、萃取时间、提取次数、固液比等对枸杞中类胡萝卜素提取得率的影响。以清除DPPH自由基为指标测定了枸杞鲜果冻干制品中类胡萝卜素清除自由基的能力。得出枸杞类胡萝卜素最佳提取工艺为微波萃取温度50℃、提取时间5min、料液比1：20、萃取3次。枸杞鲜果冻干制品中类胡萝卜素对DPPH的清除率IC50值为1.32mg/mL。说明枸杞鲜果冻干制品中的类胡萝卜素具有一定的抗氧化能力，且枸杞鲜果冻干工艺提取出的枸杞类胡萝卜素含量远高于传统的烘干法，说明真空冷冻技术对枸杞的活性成分类胡萝卜素有很好的保护作用。
채용미파췌취법우화구기중류호라복소적제취공예，동시대구기선과동간제품중류호라복소적함량급청제DPPH적능력진행료측정。이β-호라복소위대조품，채용정교실험방법연구료미파췌취온도、췌취시간、제취차수、고액비등대구기중류호라복소제취득솔적영향。이청제DPPH자유기위지표측정료구기선과동간제품중류호라복소청제자유기적능력。득출구기류호라복소최가제취공예위미파췌취온도50℃、제취시간5min、료액비1：20、췌취3차。구기선과동간제품중류호라복소대DPPH적청제솔IC50치위1.32mg/mL。설명구기선과동간제품중적류호라복소구유일정적항양화능력，차구기선과동간공예제취출적구기류호라복소함량원고우전통적홍간법，설명진공냉동기술대구기적활성성분류호라복소유흔호적보호작용。
Optimize the microwave extraction and determinate the content and antioxidant activity for carotenoid in freezing dried Lycium barbarum L. Withβ-carotene as a reference, orthogonal experiment has been designed from the reflux with microwave temperature, heating time, extracting times and the ratio of gardenia to liquor. The test showed that carotenoid concentration in freezing dried Lycium barbarum L. was extracted highest when microwave extracting three times, heat time 5 minutes, heating temperature 50℃. The inhibitory rate of DPPH scavenging activity(IC50=1.32mg/mL) was analyzed for carotenoid in freezing dried Lycium barbarum L..