CAJ | 학술논문

目的：观察颈总动脉内膜损伤后自噬的动态变化。方法40只雄性SD大鼠利用简单随机抽样法分为假手术组（n=20）和模型组(n=20)。假手术组依次分离颈部皮肤、皮下组织，暴露左侧颈总动脉后直接缝合皮肤，模型组行左侧颈总动脉内膜剥脱术。假手术组及模型组在3 d、7 d分别用western blot、免疫荧光检测颈总动脉中Beclin-1、微管相关蛋白1轻链3(microtubule associated protein 1 light chain 3，LC3)的表达；并在相同的时间点用western blot检测颈总动脉中P62的表达。结果 western blot结果显示，与假手术组相比，模型组Be-clin-1及LC3的表达于3 d、7 d增高，其中第7 d增高明显，自噬底物P62的表达于3 d、7 d下降，其中7 d下降显著。免疫荧光结果显示，与假手术组相比，模型组Beclin-1在颈总动脉中膜及内膜总的表达增高（第3 d 18.60±1.34；6.40±0.55，P=0.000，n=5；7 d 27.6±2.19；6.40±0.55，P=0.000，n=5）；模型组LC3在颈总动脉中膜及内膜总的表达增高（第3d 10.60±1.52；3.00±0.71，P=0.000，n=5；第7d 21.20±2.49；3.00±0.71，P=0.000，n=5）。结论模型组中调控自噬的Beclin-1蛋白以及自噬体形成所必需的LC3的表达增高，均表明自噬被激活，并于不同时间点呈动态变化，推测其可能参与新生内膜形成。
목적：관찰경총동맥내막손상후자서적동태변화。방법40지웅성SD대서이용간단수궤추양법분위가수술조（n=20）화모형조(n=20)。가수술조의차분리경부피부、피하조직，폭로좌측경총동맥후직접봉합피부，모형조행좌측경총동맥내막박탈술。가수술조급모형조재3 d、7 d분별용western blot、면역형광검측경총동맥중Beclin-1、미관상관단백1경련3(microtubule associated protein 1 light chain 3，LC3)적표체；병재상동적시간점용western blot검측경총동맥중P62적표체。결과 western blot결과현시，여가수술조상비，모형조Be-clin-1급LC3적표체우3 d、7 d증고，기중제7 d증고명현，자서저물P62적표체우3 d、7 d하강，기중7 d하강현저。면역형광결과현시，여가수술조상비，모형조Beclin-1재경총동맥중막급내막총적표체증고（제3 d 18.60±1.34；6.40±0.55，P=0.000，n=5；7 d 27.6±2.19；6.40±0.55，P=0.000，n=5）；모형조LC3재경총동맥중막급내막총적표체증고（제3d 10.60±1.52；3.00±0.71，P=0.000，n=5；제7d 21.20±2.49；3.00±0.71，P=0.000，n=5）。결론모형조중조공자서적Beclin-1단백이급자서체형성소필수적LC3적표체증고，균표명자서피격활，병우불동시간점정동태변화，추측기가능삼여신생내막형성。
Objective To investigate the dynamic levels of autophagy after intimal injury of carotid artery. Meth-ods In this study ,40 male SD rats were randomly assigned to operated(n=20)and control groups(n=20). Balloon inju-ry was induced in the left carotid artery in operated groups .Rats in control groups just received carotid artery exposure without injury. Western blot was used to detect the levels of Beclin-1, LC3 and p62 at the third and seventh days. Immu-nofluorescence was used to examine the expression of Beclin-1 and LC3 at the third and seventh days. Results The ex-pression levels of Beclin-1 and LC3 were increased while the levels of P62 were decreased at the third and seventh days after carotid balloon injury. Beclin-1 and LC3 were present in neointima and medintima. The numbers of both Beclin-1 positive cells and LC3 positive cells were increased at the third and seventh days after carotid injury. The numbers of Be-clin-1 positive cells were 18.60 ± 1.34 in neointima and 6.40 ± 0.55 in medintima at third day, (27.6 ± 2.19 in neointima and 6.40±0.55 in medinitima at the seventh day,(all P=0.000,n=5). The numbers of LC3 positive cells were 10.60±1.52 in neointima and 3.00 ± 0.71 in medintima at third day, (P=0.000,n=5;at the seventh day 21.20 ± 2.49;3.00 ± 0.71,P=0.000,n=5). Conclusions This study domenstrates that autophagy was activated after carotid injury and the chang is dy-namic, which may contribute to neointima formation.