CAJ | 학술논문

目的：探讨养血清脑颗粒对内源性神经干细胞增殖分化的影响和神经保护机制。方法应用TTC染色和免疫荧光双标技术观察脑缺血再灌注损伤后3 d、7 d、14 d、21 d神经缺损程度、脑梗死体积、Brdu平均荧光强度值和Brdu+Nestin荧光强度测定及治疗后的影响。结果①除假手术组外，模型对照组、养血清脑低剂量组和养血清脑高剂量大鼠均出现不同程度的神经功能缺损和脑梗死，各组大鼠不同时间点神经缺损评分和脑梗死体积均不一样，差异均有统计学意义（P<0.01）；②治疗后第3、7、14、21天侧脑室下区（subventricular zone， SVZ）和海马齿状回颗粒下层（subgranular zone，SGZ）Brdu阳性细胞或Nestin阳性细胞其平均荧光强度值不同时间点间比较均有统计学差异（P<0.01）。低、高剂量组Brdu阳性细胞平均荧光强度值和Brdu+Nestin免疫双标荧光强度值均高于模型组（P<0.01）。结论养血清脑颗粒能促进缺血再灌注脑损伤大鼠神经功能恢复减轻脑梗死体积，对脑缺血再灌注损伤后神经干细胞增殖分化有促进作用。
목적：탐토양혈청뇌과립대내원성신경간세포증식분화적영향화신경보호궤제。방법응용TTC염색화면역형광쌍표기술관찰뇌결혈재관주손상후3 d、7 d、14 d、21 d신경결손정도、뇌경사체적、Brdu평균형광강도치화Brdu+Nestin형광강도측정급치료후적영향。결과①제가수술조외，모형대조조、양혈청뇌저제량조화양혈청뇌고제량대서균출현불동정도적신경공능결손화뇌경사，각조대서불동시간점신경결손평분화뇌경사체적균불일양，차이균유통계학의의（P<0.01）；②치료후제3、7、14、21천측뇌실하구（subventricular zone， SVZ）화해마치상회과립하층（subgranular zone，SGZ）Brdu양성세포혹Nestin양성세포기평균형광강도치불동시간점간비교균유통계학차이（P<0.01）。저、고제량조Brdu양성세포평균형광강도치화Brdu+Nestin면역쌍표형광강도치균고우모형조（P<0.01）。결론양혈청뇌과립능촉진결혈재관주뇌손상대서신경공능회복감경뇌경사체적，대뇌결혈재관주손상후신경간세포증식분화유촉진작용。
Objective To investigate the effects of Yangxueqingnao Granule on the proliferation and differentiation of endogenous neural stem cells and their neuroprotective mechanism after brain ischemia. Methods Cerebral ischemia was induced in rats by temporary middle cerebral artery occlusion. Ischemic animals received Yangxue Qingnao Granule at high and low doses at days 3,7, 14,and 21 after MCAO. Neurological score and TTC staining were used to assess the neurological deficits and the volume of cerebral infarction, respectively. Immunohistochemistry was used to detect Brdu and Nestin expression in the subventricular zone(SVZ)and hippocampal dentate gyrus subgranular zone(SGZ)region. Results The rats in MCAO control group, low dose group and high dose group showed varying degrees of neurological def-icits and the volume of cerebral infarctions. The neurological defect scores and volume of cerebral infarction were signifi-cantly different at different time points in each group ( P<0.01). 2. The mean fluorescence intensity values of Brdu posi-tive cells or the Nestin positive cells in SVZ and SGZ regions were significantly different ( P< 0.01) at different time points after treatment for 3,7,14 or 21 days. The mean fluorescence intensity values of Brdu positive cells or the Nestin positive cells in SVZ and SGZ regions were significant different among low dose group, high dose group and MCAO con-trol group. The mean fluorescence intensity values of Brdu positive cells or the Nestin positive cells in SVZ and SGZ re-gions were significantly different among low dose group, High dose group and control group. High dose group significantly increased the mean fluorescence intensity values of Brdu positive cells or the Nestin positive cells in SVZ and SGZ re-gions. Conculsions Yangxue Qingnao Granule is able to promote the functional recovery and reduce the volume of cere-bral infarction in ischemic rats. Yangxue Qingnao Granule can enhance the proliferation and differentiation of endoge-nous neural stem cells in ischemic rats.