CAJ | 학술논문

目的：探讨氯沙坦钾对糖尿病肾病（diabetic nephropathy，DN）大鼠肾组织 p - JAK2、p - STAT3及 VEGF 表达的影响。方法：健康雄性 SD 大鼠30只随机分为正常对照组（C 组）、DN 模型组（DN 组）、氯沙坦钾组（L 组）。采用单次腹腔内注射链脲佐菌素（streptozotocin，STZ）法建立 DN 大鼠模型，周期12周。实验12周末检测大鼠血糖、Scr、BUN、24 h 尿蛋白定量；光镜及电镜下观察大鼠肾组织病理改变；采用免疫组化方法检测大鼠肾组织 p - JAK2、p - STAT3、VEGF 表达。结果：实验12周末，模型组大鼠血糖、Scr、BUN、24 h 尿蛋白定量均高于对照组（P ﹤0．05），肾组织中 p - JAK2、p - STAT3、VEGF 表达均高于对照组（P ﹤0．05）；氯沙坦钾组大鼠 Scr、BUN、24 h 尿蛋白定量均低于模型组（P ﹤0．05），肾组织 p - JAK2、p -STAT3、VEGF 表达均低于模型组（P ﹤0．05）。结论：氯沙坦钾可能部分通过调控 p - JAK2、p - STAT3及 VEGF 的表达而发挥肾脏保护作用。
목적：탐토록사탄갑대당뇨병신병（diabetic nephropathy，DN）대서신조직 p - JAK2、p - STAT3급 VEGF 표체적영향。방법：건강웅성 SD 대서30지수궤분위정상대조조（C 조）、DN 모형조（DN 조）、록사탄갑조（L 조）。채용단차복강내주사련뇨좌균소（streptozotocin，STZ）법건립 DN 대서모형，주기12주。실험12주말검측대서혈당、Scr、BUN、24 h 뇨단백정량；광경급전경하관찰대서신조직병리개변；채용면역조화방법검측대서신조직 p - JAK2、p - STAT3、VEGF 표체。결과：실험12주말，모형조대서혈당、Scr、BUN、24 h 뇨단백정량균고우대조조（P ﹤0．05），신조직중 p - JAK2、p - STAT3、VEGF 표체균고우대조조（P ﹤0．05）；록사탄갑조대서 Scr、BUN、24 h 뇨단백정량균저우모형조（P ﹤0．05），신조직 p - JAK2、p -STAT3、VEGF 표체균저우모형조（P ﹤0．05）。결론：록사탄갑가능부분통과조공 p - JAK2、p - STAT3급 VEGF 적표체이발휘신장보호작용。
Objective:To investigate the effect of Losartan Potassium on p - JAK2,p - STAT3 and VEGF of renal tissue in the diabetic nephropathy rats. Methods:30 SD male rats were divided randomly into control group(group C),diabetic nephropathy group(group DN),Losartan Potassium group(group L). The rats were being intraperitoneal injected with STZ(1% ,45 mg/ kg)to establish diabetic nephropathy rats models,test period 12 weeks. Blood glucose,Scr,BUN,24 - hour urinary protein were measured at the 12th. The renal pathological changes were detected by light microscope and electron microscopy. The expression of p - JAK2, p - STAT3 and VEGF were detected by immunohistochemistry staining sections. Results:After establishing mode for 12 weeks,com-pared with group C,group DN had significant elevated blood glucose,24 - hour urinary protein,Scr,BUN(P ﹤ 0. 05),and immuno-histochemistry staining displayed that the expression of p - JAK2,p - STAT3,VEGF remarkably increased(P ﹤ 0. 05). Compared with group DN,group L had significantly lower 24 urinary protein,Scr,BUN(P ﹤ 0. 05),the expression of p - JAK2,p - STAT3, VEGF were lower than those in group DN(P ﹤ 0. 05). Conclusion:Losartan Potassium may in part through regulating the expression of p - JAK2,p - STAT3 and VEGF play a protective role in the kidney of diabetic nephropathy rats.