结直肠癌患者血清中可溶性P-选择素和L-选择素水平及临床意义
결직장암환자혈청중가용성P-선택소화L-선택소수평급림상의의
Soluble P-selectin and L-selectin levels in serum of colorectal cancer patients and its clinical significance
  • 万方数据
    中国癌症杂志 중국암증잡지
    CHINA ONCOLOGY
    2014年 8期 599-603 ,共5页

    张梁%罗桂飞%元辉雄%韦贵将

    장량%라계비%원휘웅%위귀장

    结直肠癌%P-选择素%L-选择素 結直腸癌%P-選擇素%L-選擇素
    결직장암%P-선택소%L-선택소
    Colorectal cancer%P-selectin%L-selectin
    背景与目的：结直肠癌是常见的消化系统恶性肿瘤，严重威胁着人类的健康。虽然现代诊疗技术不断发展，但近年来结直肠癌的发病率和病死率仍呈逐年上升趋势，早期诊断和防治肝转移有助于提高结直肠癌患者的生存率，对改善其预后有重要意义。P-选择素和L-选择素是近年肿瘤研究领域倍受关注的分子，其表达量及介导的黏附作用的改变在肿瘤细胞转移中起着重要作用，但2者与结直肠癌发生及临床分期的关系鲜见报道。本研究旨在探讨结直肠癌患者手术前后血清中P-选择素和L-选择素水平高低与临床病理特征的相关性。方法：选择132例结直肠癌患者及与其性别年龄相匹配的100名健康体检者。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay，ELISA)检测132例结直肠癌患者手术前后血清中P-选择素和L-选择素浓度，100名健康体检者为对照组，结合临床病理特征对P-选择素和L-选择素水平的变化进行分析。结果：结直肠癌患者术前血清P-选择素和L-选择素水平显著高于对照组(75.2±13.3vs 26.6±9.6，89.2±12.7vs 33.9±8.3，P<0.05)；术后1周P-选择素和L-选择素水平较术前没有明显下降(63.8±11.1vs 75.2±13.3，71.5±10.9vs 89.2±12.7，P>0.05)；而术后3个月则明显下降，与术前比较差异有统计学意义(34.8±10.4 vs 75.2±13.3，40.1±9.5vs 89.2±12.7，P<0.01)；本组结直肠癌患者，临床分期越高，P-选择素和L-选择素水平越高，其水平与肿瘤浸润深度、Dukes分期、组织学分级和淋巴结转移相关，而与年龄、性别及肿瘤部位无明显相关(P>0.05)；同时，同一结直肠癌患者血清中P-选择素和L-选择素的表达无显著相关(r=4.114， P>0.05)。结论：血清中P-选择素和L-选择素的水平在一定程度上反映了结直肠癌的侵袭、浸润和淋巴结转移的程度，可作为预测结直肠癌发展及预后的重要指标。
    배경여목적：결직장암시상견적소화계통악성종류，엄중위협착인류적건강。수연현대진료기술불단발전，단근년래결직장암적발병솔화병사솔잉정축년상승추세，조기진단화방치간전이유조우제고결직장암환자적생존솔，대개선기예후유중요의의。P-선택소화L-선택소시근년종류연구영역배수관주적분자，기표체량급개도적점부작용적개변재종류세포전이중기착중요작용，단2자여결직장암발생급림상분기적관계선견보도。본연구지재탐토결직장암환자수술전후혈청중P-선택소화L-선택소수평고저여림상병리특정적상관성。방법：선택132례결직장암환자급여기성별년령상필배적100명건강체검자。채용매련면역흡부시험(enzyme-linked immunosorbent assay，ELISA)검측132례결직장암환자수술전후혈청중P-선택소화L-선택소농도，100명건강체검자위대조조，결합림상병리특정대P-선택소화L-선택소수평적변화진행분석。결과：결직장암환자술전혈청P-선택소화L-선택소수평현저고우대조조(75.2±13.3vs 26.6±9.6，89.2±12.7vs 33.9±8.3，P<0.05)；술후1주P-선택소화L-선택소수평교술전몰유명현하강(63.8±11.1vs 75.2±13.3，71.5±10.9vs 89.2±12.7，P>0.05)；이술후3개월칙명현하강，여술전비교차이유통계학의의(34.8±10.4 vs 75.2±13.3，40.1±9.5vs 89.2±12.7，P<0.01)；본조결직장암환자，림상분기월고，P-선택소화L-선택소수평월고，기수평여종류침윤심도、Dukes분기、조직학분급화림파결전이상관，이여년령、성별급종류부위무명현상관(P>0.05)；동시，동일결직장암환자혈청중P-선택소화L-선택소적표체무현저상관(r=4.114， P>0.05)。결론：혈청중P-선택소화L-선택소적수평재일정정도상반영료결직장암적침습、침윤화림파결전이적정도，가작위예측결직장암발전급예후적중요지표。
    Background and purpose:Colorectal cancer is a kind of common digestive malignancies, which seriously threaten the human health. Although modern diagnostic and treatment technology has developed rapidly, the incidence and mortality of colorectal cancer continue to show an increasing tendency in recent years, and early diagnosis and prevention of colorectal cancer liver metastases are important to increase the survival rate of patients and to improve the prognosis. P-selectin and L-selectin is attracting much attention in cancer research ifeld recently, and the change of their expression and mediated adhesion play an important role in tumor cell metastasis, but their relationship between the occurrence and clinical stage of colorectal cancer rarely reported. This study aimed to explore whether the serum P-selectin and L-selectin levels of colorectal cancer patients were correlated with clinical and pathological features and the situation before and after surgery.Methods:A total number of 132 cases of colorectal cancer patients and 100 healthy subjects with gender and age-matched were enrolled. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum P-selectin and L-selectin concentrations in 132 cases before and after surgery, 100 healthy subjects were enrolled as the control group, the P-selectin and L-selectin levels were analyzed combined with theclinical and pathological features.Results:Serum P-selectin and L-selectin levels in patients with colorectal cancer before surgery were signiifcantly higher than those in the healthy control group (75.2±13.3vs 26.6±9.6, 89.2±12.7vs 33.9±8.3,P<0.05) ; Compared with the patients before surgery, 1 week after surgery, the P-selectin and L-selectin levels were not significantly decreased (63.8±11.1vs 75.2±13.3, 71.5±10.9vs 89.2±12.7,P>0.05); however, significantly decreased after 3 months (34.8±10.4vs 75.2±13.3, 40.1±9.5vs 89.2±12.7,P<0.01); This paper studied patients with colorectal cancer, the higher clinical stage, higher P-selectin and L-selectin levels, and their expression levels with tumor invasion depth, Dukes stage, histological grade and lymph node metastasis were related, but were not correlated with age, gender and tumor site. Meanwhile, P-selectin and L-selectin levels were not signiifcantly correlated in the same serum of patients with colorectal cancer (r=4.114,P>0.05).Conclusion:Serum P-selectin and L-selectin levels to some extent relfect the invasion of colorectal cancer, the degree of inifltration and lymph node metastasis, and which can be an important indicator in the development and prognosis of colorectal cancer.
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