CAJ | 학술논문

目的：金属假体以其良好的性质在关节置换中得到广泛应用。文中探讨金属假体使用过程中产生的金属颗粒刺激对人单核细胞内氧化应激水平的影响。方法抽取15名健康志愿者外周血，离心后吸取单个核细胞，将细胞进行体外培养，分别加入将钴颗粒、铬颗粒、铁颗粒、钛颗粒形成钴、铬、铁、钛颗粒组，并使颗粒浓度最终约达到20×109个/mL。对照组加入等渗盐水。分别在刺激后6、12、24、36、48 h检测细胞内活性氧（ROS）、丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽氧化物酶（ GSH）含量。结果单核细胞与金属颗粒共培养6、12、24、36、48 h后，经统计学分析各颗粒组MDA、ROS水平均高于对照组水平（P＜0．05）。钴、铬、铁颗粒组MDA、ROS水平均高于钛颗粒组（P＜0．05）；各颗粒组SOD、GSH水平均低于对照组（P＜0．05）。钴、铬、铁颗粒组SOD、GSH水平均低于钛颗粒组（P＜0．05）。结论金属颗粒能导致体外培养的单核细胞细胞内氧化应激水平升高，降低细胞内的抗氧化物酶水平，钴、铬、铁颗粒的诱导活性强于钛颗粒。氧化应激在金属颗粒介导的骨溶解中可能起到了关键作用。
목적：금속가체이기량호적성질재관절치환중득도엄범응용。문중탐토금속가체사용과정중산생적금속과립자격대인단핵세포내양화응격수평적영향。방법추취15명건강지원자외주혈，리심후흡취단개핵세포，장세포진행체외배양，분별가입장고과립、락과립、철과립、태과립형성고、락、철、태과립조，병사과립농도최종약체도20×109개/mL。대조조가입등삼염수。분별재자격후6、12、24、36、48 h검측세포내활성양（ROS）、병이철（MDA）、초양화물기화매（SOD）、곡광감태양화물매（ GSH）함량。결과단핵세포여금속과립공배양6、12、24、36、48 h후，경통계학분석각과립조MDA、ROS수평균고우대조조수평（P＜0．05）。고、락、철과립조MDA、ROS수평균고우태과립조（P＜0．05）；각과립조SOD、GSH수평균저우대조조（P＜0．05）。고、락、철과립조SOD、GSH수평균저우태과립조（P＜0．05）。결론금속과립능도치체외배양적단핵세포세포내양화응격수평승고，강저세포내적항양화물매수평，고、락、철과립적유도활성강우태과립。양화응격재금속과립개도적골용해중가능기도료관건작용。
Objective To investigate the effects of metal particles produced by metal prosthesis on oxidative stress levels in human mononuclear cells . Methods Mononuclear cells obtained from peripheral blood of 15 healthy volunteers .Coculture mononu-clear cells with iron particles , cobalt particles , chromium particles , titanium particles and physiological saline , respectively .Levels of reactive oxygen species ( ROS) , malondialdehyde ( MDA) , superoxide dismutase ( SOD) and glutathione peroxidase ( GSH) were as-sessed in mononuclear cells at 6 h, 12 h, 24 h, 36 h, 48 h, respectively . Results Levels of ROS and MDA were higher in the parti-cle groups than that in the control group at 6 h, 12 h, 24 h, 36 h, 48 h, respectively (P<0.05).Levels of ROS and MDA were higher in cobalt particle, chromium particle and iron particle groups than that in the titanium particle group (P<0.05).Levels of SOD and GSH were lower in particle groups than that in the control group (P<0.05).Levels of SOD and GSH were lower in cobalt particle , chromium particle and iron particle groups than that in the titanium particle group (P<0.05). Conclusion Oxidative stress levels increased and anti-oxidant levels decreased in mononuclear cells when cocultured with metal debris .Cobalt particles , chromium parti-cles and iron particles induced higher changes than that in titanium particles .Oxidative stress may play an important role in metal deb-ris-mediated osteolysis .