CAJ | 학술논문

目的：前列腺癌是男性最常见的恶性肿瘤之一。文中旨在探讨VEGF-C mRNA在前列腺癌中的表达及临床病理意义，以期寻找针对前列腺癌早期诊断和预后评估的分子标记物。方法应用TaqMan探针法实时定量PCR技术检测VEGF-C mRNA在3种前列腺癌细胞系（ PC-3、DU145和LNCap）、32例前列腺癌及15例良性前列腺增生组织标本中的表达，实验数据应用2-ΔΔC t进行处理，2-ΔΔC t ＞2为VEGF-C mRNA高表达，2-ΔΔC t≤2为低表达。并分析其表达水平与临床病理参数及预后之间的关系。结果 VEGF-C mRNA在前列腺癌细胞系PC-3和DU145中高表达，相对表达量分别为153．31±26．24和194．62±41．36，在LNCap中低表达，相对表达量为1．00±0．00（P＜0．05）。在前列腺癌组织中的表达较前列腺增生明显增高（13．67±1．95 vs 11．89±1．63，P＝0．004），癌组织中VEGF-C mRNA的高表达与高Gleasson评分、淋巴结转移相关（P＝0．004，P＝0．015）。前列腺癌VEGF-C mRNA高表达者、低表达者的3年累积生存率分别为12．5％、40．0％（P＝0．033）。结论 VEGF-C mRNA可能与前列腺癌发生发展相关，可能成为判断前列腺癌预后的标志物。
목적：전렬선암시남성최상견적악성종류지일。문중지재탐토VEGF-C mRNA재전렬선암중적표체급림상병리의의，이기심조침대전렬선암조기진단화예후평고적분자표기물。방법응용TaqMan탐침법실시정량PCR기술검측VEGF-C mRNA재3충전렬선암세포계（ PC-3、DU145화LNCap）、32례전렬선암급15례량성전렬선증생조직표본중적표체，실험수거응용2-ΔΔC t진행처리，2-ΔΔC t ＞2위VEGF-C mRNA고표체，2-ΔΔC t≤2위저표체。병분석기표체수평여림상병리삼수급예후지간적관계。결과 VEGF-C mRNA재전렬선암세포계PC-3화DU145중고표체，상대표체량분별위153．31±26．24화194．62±41．36，재LNCap중저표체，상대표체량위1．00±0．00（P＜0．05）。재전렬선암조직중적표체교전렬선증생명현증고（13．67±1．95 vs 11．89±1．63，P＝0．004），암조직중VEGF-C mRNA적고표체여고Gleasson평분、림파결전이상관（P＝0．004，P＝0．015）。전렬선암VEGF-C mRNA고표체자、저표체자적3년루적생존솔분별위12．5％、40．0％（P＝0．033）。결론 VEGF-C mRNA가능여전렬선암발생발전상관，가능성위판단전렬선암예후적표지물。
Objective Prostate cancer is one of the most common cancer in males .The article was to investigate the expres-sion and clinicopathological significance of VEGF-C mRNA in prostate cancer , finding the molecular marker for early diagnosis and prognosis assessment of prostate cancer . Methods TaqMan real-time polymerase chain reaction ( PCR) analysis was used to detect the expression of VEGF-C mRNA in 3 prostate cancer cell lines(PC-3, DU145 and LNCap), 32 cases of prostate cancer (Pca) sam-ples and 15 cases of benign prostatic hyperplasia (BPH).Analysis was also made on the correlations of VEGF-C mRNA expression, clinicopathological features and prognosis . Results High levels of VEGF-C mRNA were detected in PC-3 ( 153 .31 ±26 .24 ) and DU145(194.62 ±41.36)compared to LNcap(1.00 ±0.00).The expression of VEGF-C mRNA in prostate cancer tissues was 3.43 folds higher than that in the benign prostatic hyperplasia tissues ([13.67 ±1.95] vs [11.89 ±1.63], P=0.004).The high expres-sion of VEGF-C mRNA in prostate cancer was associated with high Gleason score ( P =0.004 ) and lymph node metastasis ( P =0.015).In patients with high expression and low expression of VEGF-C mRNA, the 3-year survival rate was 12.5%and 40.0%re-spectively(P=0.033). Conclusion The VEGF-C mRNA expression may be related to the biological behavior of prostate cancer .It is suggested that VEGF-C mRNA can be used as a prognostic marker for prostate cancer .