医学研究生学报
醫學研究生學報
의학연구생학보
JOURNAL OF MEDICAL POSTGRADUATE
2014年
8期
797-801
,共5页
李建民%赵雅宁%马荣丽%窦娜%陈长香%李淑杏%张盼
李建民%趙雅寧%馬榮麗%竇娜%陳長香%李淑杏%張盼
리건민%조아저%마영려%두나%진장향%리숙행%장반
重型弥漫性脑损伤%Homer1a%凋亡%大鼠
重型瀰漫性腦損傷%Homer1a%凋亡%大鼠
중형미만성뇌손상%Homer1a%조망%대서
Severe diffuse brain injury%Homer1a%Apoptosis%Rats
目的: Homer1a是惟一在外来刺激后高表达的Homer 家族蛋白。文中观察弥漫性颅脑损伤( diffuse brain injury, DBI)大鼠海马区Homer1a蛋白表达变化、凋亡神经细胞动态变化,以探讨重型DBI大鼠海马区Homer1a表达与神经细胞丢失的关系。方法按随机数字列表法将SD大鼠分为对照组(n=35)、DBI组(n=69)。 Marmarou′s法建立大鼠DBI模型。对照组不致伤。应用光镜和电镜下观察伤后脑组织形态变化;免疫组化及Western blot法检测海马区Homer1a表达,原位缺口末端标记法(in situ nick-end labeling, TUNEL)检测神经细胞的凋亡。结果 DBI组大鼠死亡率49.3%;其海马区神经元内细胞器、轴索及毛细血管等超微结构较对照组明显受损;伤后1 h DBI 组存活神经细胞百分率较对照组显著降低[(94.4±5.6)%vs (99.4±0.6)%, P<0.05],伤后72 h降低最为显著[(54.7±33.8)% vs (99.2±0.8)%, P<0.05];Homer1a伤后1 h表达显著增高(0.136±0.024),6 h出现峰值(0.178±0.028),高表达状态持续至24 h(0.176±0.027)、48 h (0.145±0.02)和72 h(0.117±0.012)表达下降,各时间点与对照组比较差异均有统计学意义(P<0.05);TUNEL阳性细胞凋亡指数,72 h较对照组明显升高[(41.78±3.96)%vs (1.92±0.22)%, P<0.05]。相关分析显示1~24 h Homer1a蛋白表达与存活神经细胞百分率呈负相关(r=-0.726,P<0.05),与TUNEL阳性细胞数量呈正相关(r=0.738,P<0.05);24~72 h Homer1a蛋白表达与TUNEL阳性细胞数量呈负相关(r=-0.898,P<0.05),与存活神经细胞百分率呈正相关(r=0.842, P<0.05)。结论重型DBI后海马区Homer1a蛋白动态表达可反映神经细胞丢失。
目的: Homer1a是惟一在外來刺激後高錶達的Homer 傢族蛋白。文中觀察瀰漫性顱腦損傷( diffuse brain injury, DBI)大鼠海馬區Homer1a蛋白錶達變化、凋亡神經細胞動態變化,以探討重型DBI大鼠海馬區Homer1a錶達與神經細胞丟失的關繫。方法按隨機數字列錶法將SD大鼠分為對照組(n=35)、DBI組(n=69)。 Marmarou′s法建立大鼠DBI模型。對照組不緻傷。應用光鏡和電鏡下觀察傷後腦組織形態變化;免疫組化及Western blot法檢測海馬區Homer1a錶達,原位缺口末耑標記法(in situ nick-end labeling, TUNEL)檢測神經細胞的凋亡。結果 DBI組大鼠死亡率49.3%;其海馬區神經元內細胞器、軸索及毛細血管等超微結構較對照組明顯受損;傷後1 h DBI 組存活神經細胞百分率較對照組顯著降低[(94.4±5.6)%vs (99.4±0.6)%, P<0.05],傷後72 h降低最為顯著[(54.7±33.8)% vs (99.2±0.8)%, P<0.05];Homer1a傷後1 h錶達顯著增高(0.136±0.024),6 h齣現峰值(0.178±0.028),高錶達狀態持續至24 h(0.176±0.027)、48 h (0.145±0.02)和72 h(0.117±0.012)錶達下降,各時間點與對照組比較差異均有統計學意義(P<0.05);TUNEL暘性細胞凋亡指數,72 h較對照組明顯升高[(41.78±3.96)%vs (1.92±0.22)%, P<0.05]。相關分析顯示1~24 h Homer1a蛋白錶達與存活神經細胞百分率呈負相關(r=-0.726,P<0.05),與TUNEL暘性細胞數量呈正相關(r=0.738,P<0.05);24~72 h Homer1a蛋白錶達與TUNEL暘性細胞數量呈負相關(r=-0.898,P<0.05),與存活神經細胞百分率呈正相關(r=0.842, P<0.05)。結論重型DBI後海馬區Homer1a蛋白動態錶達可反映神經細胞丟失。
목적: Homer1a시유일재외래자격후고표체적Homer 가족단백。문중관찰미만성로뇌손상( diffuse brain injury, DBI)대서해마구Homer1a단백표체변화、조망신경세포동태변화,이탐토중형DBI대서해마구Homer1a표체여신경세포주실적관계。방법안수궤수자렬표법장SD대서분위대조조(n=35)、DBI조(n=69)。 Marmarou′s법건립대서DBI모형。대조조불치상。응용광경화전경하관찰상후뇌조직형태변화;면역조화급Western blot법검측해마구Homer1a표체,원위결구말단표기법(in situ nick-end labeling, TUNEL)검측신경세포적조망。결과 DBI조대서사망솔49.3%;기해마구신경원내세포기、축색급모세혈관등초미결구교대조조명현수손;상후1 h DBI 조존활신경세포백분솔교대조조현저강저[(94.4±5.6)%vs (99.4±0.6)%, P<0.05],상후72 h강저최위현저[(54.7±33.8)% vs (99.2±0.8)%, P<0.05];Homer1a상후1 h표체현저증고(0.136±0.024),6 h출현봉치(0.178±0.028),고표체상태지속지24 h(0.176±0.027)、48 h (0.145±0.02)화72 h(0.117±0.012)표체하강,각시간점여대조조비교차이균유통계학의의(P<0.05);TUNEL양성세포조망지수,72 h교대조조명현승고[(41.78±3.96)%vs (1.92±0.22)%, P<0.05]。상관분석현시1~24 h Homer1a단백표체여존활신경세포백분솔정부상관(r=-0.726,P<0.05),여TUNEL양성세포수량정정상관(r=0.738,P<0.05);24~72 h Homer1a단백표체여TUNEL양성세포수량정부상관(r=-0.898,P<0.05),여존활신경세포백분솔정정상관(r=0.842, P<0.05)。결론중형DBI후해마구Homer1a단백동태표체가반영신경세포주실。
(DBI) in rats by observation on the changes of Homer 1a expression and apoptotic nerve cells . Methods Spraque-Dawlley(SD) rats were randomly ( random number ) divided into control group and severe DBI group .DBI rat model was established according to the de-scription of Marmarou′s diffused brain injury .No injury was done on control group .The changes of neuron pathology were observed by light microscopy and electron microscope .The expression of Homer1a was observed by immunohistochemistry and western blot .The quan-tity of apoptotic cells was measured by terminal deoxynucleotidyl transfernase medicated nick end labeling ( TUNEL) method. Results The death rate of rats in severe DBI group was 49.3%.Compared with the control group , the ultrastructures in hippocampal neurons in-cluding organelle , axonal and capillary were damaged seriously after injury , the survival rate of nerve cells decreased significantly at 1 h after injury ([99.4 ±0.6]%vs [94.4 ±5.6]%, P<0.05), and peaked at 72 h ([99.2 ±0.8]%vs [54.7 ±33.8]%, P<0 .05) in DBI group.The expression of Homer1a protein increased significantly at 1 h after injury(0 .136 ±0.024 )and peaked at6 h(0.178 ± 0.028) and maintained to 24 h (0.176 ±0.027), while decreased at 48 h (0.145 ±0.02)and 72 h (0.117 ±0.012) in DBI group;the expression of Homer 1a was obviously higher at each time point in DBI group than that in control group (P <0.05).The apoptoticindex of TUNEL positive cells increased at 6 h and demonstrated significant difference at 72h in comparison to control group ([41.78 ±3 .96]%vs [1.92 ±0.22]%, P<0.05).The correlation analysis indicated that Homer1a expression from 1~24 h and 24 h~72 h was related to the survival rate of nerve cells ( r=-0.726, P<0.05; r=0.842, P<0.05) and the quantity of TUNE positive cells(r=0.738, P<0.0;5 r=-0.898, P<0.05). Conclusion The dynamic expression of Homer1a in hippocampus after severe DBI can reflect nerve cell loss.