CAJ | 학술논문

目的：神经系统疾病与神经细胞的凋亡密切相关。文中旨在探讨牛磺酸通过活化Caspase 9对海马神经元细胞凋亡的抑制作用，进而探讨牛磺酸对神经系统的保护作用及其机制。方法海马神经元细胞分为4组：对照组、损伤凋亡组、牛磺酸低剂量保护组、牛磺酸高剂量保护组。监测各组细胞生长状态，MTT监测各组细胞凋亡状态，免疫荧光及蛋白印迹法测定Caspase 9在各组中的表达水平。结果与对照组比较，损伤凋亡组海马神经元细胞生长不良，MTT实验示损失凋亡组细胞活力（A值为0．102±0．025）明显低于对照组（A值为0．643±0．013），低、高剂量干预组细胞活力（A值分别为0．504±0．072、0．452±0．029）明显提高（P＜0．05）；免疫荧光测定示损伤凋亡组Caspase 9活化明显增高（A值为61386．8±10083．6），对照组（A值为4502．2±2518．1）及牛磺酸低、高剂量保护组（A值分别为20077．4±4187．5和13976．2±7044．1）活化较低（P＜0．05）；蛋白印迹法示损伤凋亡组Caspase 9表达（A值为1．23）较对照组（A值为0．17）及低、高剂量保护组（A值分别为0．21和0．19），明显升高（P＜0．05）。结论牛磺酸可抑制Caspase 9的活化，对神经细胞有较好的保护作用。
목적：신경계통질병여신경세포적조망밀절상관。문중지재탐토우광산통과활화Caspase 9대해마신경원세포조망적억제작용，진이탐토우광산대신경계통적보호작용급기궤제。방법해마신경원세포분위4조：대조조、손상조망조、우광산저제량보호조、우광산고제량보호조。감측각조세포생장상태，MTT감측각조세포조망상태，면역형광급단백인적법측정Caspase 9재각조중적표체수평。결과여대조조비교，손상조망조해마신경원세포생장불량，MTT실험시손실조망조세포활력（A치위0．102±0．025）명현저우대조조（A치위0．643±0．013），저、고제량간예조세포활력（A치분별위0．504±0．072、0．452±0．029）명현제고（P＜0．05）；면역형광측정시손상조망조Caspase 9활화명현증고（A치위61386．8±10083．6），대조조（A치위4502．2±2518．1）급우광산저、고제량보호조（A치분별위20077．4±4187．5화13976．2±7044．1）활화교저（P＜0．05）；단백인적법시손상조망조Caspase 9표체（A치위1．23）교대조조（A치위0．17）급저、고제량보호조（A치분별위0．21화0．19），명현승고（P＜0．05）。결론우광산가억제Caspase 9적활화，대신경세포유교호적보호작용。
Objective Neurological diseases are closely associated with the apoptosis of neuronal cells .This article aims to study the inhibitory effect of taurine on the apoptosis of hippocampal neurons by activating Caspase 9 as well as its protective effect on the nervous system and its mechanisms . Methods Mouse hippocampal neuronal cells were randomly divided into four groups:control, injury and apoptosis, low-dose taurine protection, and high-dose taurine protection.The proliferation of the neuronalcells was observed, their apoptosis examined by MTT colorimetric assay, and the expression of Caspase 9 in different groups detected by immunofluorescence and Western blot. Results The injury and apoptosis group showed a poor proliferation of the hippocampal neuronal cells and decreased cell viability (A=0.102 ±0.025), significantly lower than the control group (relative A=0.643 ± 0.013), the low-dose taurine group (relative A=0.504 ±0.072), and the high-dose taurine group (relative A=0.452 ±0.029) ( all P<0 .05 ) .Immunofluorescence assay revealed significantly increased Caspase 9 activation in the injury and apoptosis group (A=61386.8 ±10083.6) compared with the control (A=4502.2 ±2518.1), the low-dose taurine (A=20077.4 ±4187.5), and the high-dose taurine group (A=13976.2 ±7044.1) (all P<0.05).Western blot showed a remarkably higher expression of Caspase 9 in in the injury and apoptosis group (A=1.23) than in the control (relative A=0.17), the low-dose taurine (A=0.21), and the high-dose taurine group (A=0.19) (all P<0.05). Conclusion Taurine can protect neuronal cells by inhibi-ting Caspase 9 activation.