西部中医药
西部中醫藥
서부중의약
GANSU JOURNAL OF TRADITIONAL CHINESE MEDICINE
2014年
8期
14-15,16
,共3页
吴紫糊%高效液相色谱法%左旋紫草素%含量测定
吳紫糊%高效液相色譜法%左鏇紫草素%含量測定
오자호%고효액상색보법%좌선자초소%함량측정
WuZi cataplasm%high performance liquid chromatography%shikonin%content determination
目的：建立吴紫糊中左旋紫草素的测定方法。方法：高效液相色谱法，色谱条件色谱柱为安捷伦C 18柱(4.6 mm×250 mm，5μm)，流动相为甲醇-0.1%磷酸盐缓冲液(80∶20)，检测波长516 nm，柱温25℃，流速10 mL/min。结果：进样量在8.2～41.0μg/mL范围内，线性关系良好(r=0.9999)平均回收率为101.30％(RSD为1.55％， n=9)。结论：高效液相色谱法测定吴紫糊中左旋紫草素含量结果准确、重现性好，适用于吴紫糊的质量控制。
目的：建立吳紫糊中左鏇紫草素的測定方法。方法：高效液相色譜法，色譜條件色譜柱為安捷倫C 18柱(4.6 mm×250 mm，5μm)，流動相為甲醇-0.1%燐痠鹽緩遲液(80∶20)，檢測波長516 nm，柱溫25℃，流速10 mL/min。結果：進樣量在8.2～41.0μg/mL範圍內，線性關繫良好(r=0.9999)平均迴收率為101.30％(RSD為1.55％， n=9)。結論：高效液相色譜法測定吳紫糊中左鏇紫草素含量結果準確、重現性好，適用于吳紫糊的質量控製。
목적：건립오자호중좌선자초소적측정방법。방법：고효액상색보법，색보조건색보주위안첩륜C 18주(4.6 mm×250 mm，5μm)，류동상위갑순-0.1%린산염완충액(80∶20)，검측파장516 nm，주온25℃，류속10 mL/min。결과：진양량재8.2～41.0μg/mL범위내，선성관계량호(r=0.9999)평균회수솔위101.30％(RSD위1.55％， n=9)。결론：고효액상색보법측정오자호중좌선자초소함량결과준학、중현성호，괄용우오자호적질량공제。
Objective:To establish the determination method for shikonin in WuZi cataplasm. Methods:High performance liquid chromatography (HPLC), chromatographic condition, chromatographic column:agilent C 18 col-umn (4.6 mm ×250 mm, 5 μm), mobile phase:methanol-0.1%phosphate buffer solution (80:20), detection wave-length:516nm, column temperature:25℃, flow rate was 10mL/min. Results:Sample size was in the range between 8.2 and 41.0 μg/mL, linear relationship was better (r=0.999 9), average recovery rate was 101.30% (RSD was 1.55%, n=9). Conclusion: HPLC determining the contents of shikonin in WuZi cataplasm is accurate and repro-ducible, which could be applied to quality control of WuZi cataplasm.
