CAJ | 학술논문

为全面了解牛支原体NM2012株的基因序列，研究其潜在的致病机制及其复制、转录、翻译过程的调控机制，采用体外大量培养，酚-氯仿法提取牛支原体NM2012株基因组DNA，并送往北京华大基因公司进行全基因组测序注释。结果显示，牛支原体NM2012株基因组大小为993483bp，GC含量为29．26％，测序组装后共产生3个scaffolds，24个contigs。基因组组分分析后发现，NM2012株的基因组含有885个基因，总长度为834042bp，基因平均长度为942bp，占基因组全长的83．95％。串联重复序列共79个，总长为9203bp，占基因组全长的0．9263％。小卫星序列53个，微卫星序列5个，tRNA34个，rRNA4个。牛支原体NM2012株16SrRNA序列与支原体属致牛羊病主要菌株构建进化树进一步证实NM2012株为牛支原体，为后期进一步研究牛支原体提供依据。
위전면료해우지원체NM2012주적기인서렬，연구기잠재적치병궤제급기복제、전록、번역과정적조공궤제，채용체외대량배양，분-록방법제취우지원체NM2012주기인조DNA，병송왕북경화대기인공사진행전기인조측서주석。결과현시，우지원체NM2012주기인조대소위993483bp，GC함량위29．26％，측서조장후공산생3개scaffolds，24개contigs。기인조조분분석후발현，NM2012주적기인조함유885개기인，총장도위834042bp，기인평균장도위942bp，점기인조전장적83．95％。천련중복서렬공79개，총장위9203bp，점기인조전장적0．9263％。소위성서렬53개，미위성서렬5개，tRNA34개，rRNA4개。우지원체NM2012주16SrRNA서렬여지원체속치우양병주요균주구건진화수진일보증실NM2012주위우지원체，위후기진일보연구우지원체제공의거。
The purpose of this test was to understand the genetic sequence of Mycoplasma bovis NM2012 strain ,and study its potential pathogenesis and its regulation mechanisms of replication ,transcription and translation .NM2012 strain in vitro and its genomic DNA was extracted by phenol-chloroform extraction method and sent to the Beijing Genomics Institute for whole genome sequence .The result showed that the genome size of Mycoplasma bovis NM2012 strain is 993 483 bp ,GC content is 29 .26% ,a total of three scaffolds and 24 contigs .Genome analysis found that Mycoplasma bovis NM2012 genome contains 885 genes ,total length is 834 042 bp ,average length is 942 bp ,representing 83 .95% of the full length ge-nome .Tandem repeats is 79 ,total length is 9 203 bp ,accounting for 0 .926 3% of full-length genome . There are 53 small satellite sequences ,5 microsatellite sequences ,34 tRNA and 4 rRNA .By constructing phylogenetic tree with 16 S rRNA sequence from sequenced Mycoplasma bovis NM2012 strain and Myco-plasma which mainly infecting cattle and sheep ,we further confirmed that NM2012 strain is Mycoplasma bovis .The study provides basis for further research on Mycoplasma bovis .