CAJ | 학술논문

目的：探究胰高血糖素样肽1(GLP 1)受体激动剂 Exendin 4(Ex 4)在大鼠缺血性室性心律失常中的作用及机制。方法40只雄性SD大鼠随机分为4组。缺血组(MI)；Ex 4+缺血组(MI + Ex 4)；5羟基葵酸+ Ex 4+缺血组(MI Ex 45 HD)；5羟基葵酸+缺血组(MI 5 HD)。各组在制作 MI模型前分别给予生理盐水、Ex 4+5 HD、5 HD预处理。持续心电监护,观察缺血30 min内各组室性心动过速(VT)和心室颤动(VF)发生率、VT+VF持续时间、发生次数和室性心律失常的严重程度。结果 Ex 4显著减少大鼠心肌缺血30 min发生率(P<0.05)；与心肌缺血组比较,Ex 4可以有效减少室速+室颤的持续时间、发生次数和心律失常的严重程度有关(P<0.05)；5 HD可以有效消除 Ex 4减少室速+室颤的持续时间、发生次数和心律失常的严重程度有关(P<0.05)。结论胰高血糖素样肽1受体激动剂 Ex 4有效减轻大鼠缺血性室性心律失常,而这一保护作用可能与线粒体KATP通道的信号转导相关。
목적：탐구이고혈당소양태1(GLP 1)수체격동제 Exendin 4(Ex 4)재대서결혈성실성심률실상중적작용급궤제。방법40지웅성SD대서수궤분위4조。결혈조(MI)；Ex 4+결혈조(MI + Ex 4)；5간기규산+ Ex 4+결혈조(MI Ex 45 HD)；5간기규산+결혈조(MI 5 HD)。각조재제작 MI모형전분별급여생리염수、Ex 4+5 HD、5 HD예처리。지속심전감호,관찰결혈30 min내각조실성심동과속(VT)화심실전동(VF)발생솔、VT+VF지속시간、발생차수화실성심률실상적엄중정도。결과 Ex 4현저감소대서심기결혈30 min발생솔(P<0.05)；여심기결혈조비교,Ex 4가이유효감소실속+실전적지속시간、발생차수화심률실상적엄중정도유관(P<0.05)；5 HD가이유효소제 Ex 4감소실속+실전적지속시간、발생차수화심률실상적엄중정도유관(P<0.05)。결론이고혈당소양태1수체격동제 Ex 4유효감경대서결혈성실성심률실상,이저일보호작용가능여선립체KATP통도적신호전도상관。
Objective To investigate whether glucagon like peptide 1(GLP 1)and its receptor agonist exendin 4 (Ex 4) could provide benefi cial effects for ischemia induced ventricular arrhythmias in rats.Methods Forty SD male rats (250 g to 300 g) were randomly divided into four groups:Myocardial infarct group (MI group,n=10),myocardial ischemia plus Ex 4 group (MI+Ex 4 group,n=10),myocardial ischemia plus Ex 4 plus 5 hydroxydecanoic acid group (MI+Ex 4+5 HD group,n=10),myocardi-al ischemia plus 5 hydroxydecanoic acid group (MI+5 HD group,n=10).During 30 minute ischemia,the incidence of ventricular tachycardia (VT)and ventricular fibrillation (VF),duration of VT+VF,number of VT+VF,and severity of arrhythmias were mo-nitored and analyzed.Results During the 30 minute ischemia,Ex 4 significantly reduced the incidence of VF (P<0.05).The dura-tion of VT+VF,the number of VT+VF episodes,and the severity of arrhythmias were all significantly reduced by Ex 4 compared to those in MI group (P<0.05 for all).Administration of 5 HD abolished the protective effects of Ex 4 on VF incidence,the dura-tion of VT+VF,the number of VT+VF episodes,and the severity of arrhythmias (P<0.05 for all).Conclusion Ex 4 could atten-uate ischemia induced ventricular arrhythmias in rats in which mitochondrial KATP channels might be involved.