CAJ | 학술논문

目的：观察脓毒症大鼠血脑屏障通透性及脑组织胞质附着蛋白（ ZO-1）的变化。方法50只健康SD大鼠随机分为假手术组（ n＝10）及脓毒症模型组（ n＝40），脓毒症模型组又分为脓毒症制模后6、12、24、48 h 4个亚组，每组10只，采用盲肠结扎穿孔（ CLP）法制作脓毒症模型。于相应时间点处死取材，伊文思蓝（ EB）外渗分光光度分析法检测血脑屏障通透性；RT-PCR法测定脑组织ZO-1 mRNA表达、Western-blot免疫印迹方法检测ZO-1表达。结果脓毒症组各时间点大鼠脑内EB含量均明显高于假手术组（ P＜0．01）；脓毒症各时间点组大鼠脑内ZO-1 mRNA、ZO-1的表达均明显下降，与假手术组比较差异有统计学意义（P＜0．01），且随着脓毒症时间延长下降越明显。结论脓毒症大鼠脑内ZO-1 mRNA、ZO-1的表达均显著下降，可能是脓毒症血脑屏障受损的机制之一。
목적：관찰농독증대서혈뇌병장통투성급뇌조직포질부착단백（ ZO-1）적변화。방법50지건강SD대서수궤분위가수술조（ n＝10）급농독증모형조（ n＝40），농독증모형조우분위농독증제모후6、12、24、48 h 4개아조，매조10지，채용맹장결찰천공（ CLP）법제작농독증모형。우상응시간점처사취재，이문사람（ EB）외삼분광광도분석법검측혈뇌병장통투성；RT-PCR법측정뇌조직ZO-1 mRNA표체、Western-blot면역인적방법검측ZO-1표체。결과농독증조각시간점대서뇌내EB함량균명현고우가수술조（ P＜0．01）；농독증각시간점조대서뇌내ZO-1 mRNA、ZO-1적표체균명현하강，여가수술조비교차이유통계학의의（P＜0．01），차수착농독증시간연장하강월명현。결론농독증대서뇌내ZO-1 mRNA、ZO-1적표체균현저하강，가능시농독증혈뇌병장수손적궤제지일。
Obj ective To observe the changes on blood -brain barrier permeability and brain expression of ZO -1 in sepsis rats.Methods Fifty SD rats were divided into sham operation group (n=10) and model group (n =40).The model rats were further divided into 6, 12, 24, 48 h subgroups (n=10).The sepsis rat model was developed by CLP .The blood-brain barrier permeability was detected by spectrophotometric analysis of EB .The expression of ZO -1 mRNA and protein was detected by RT-PCR and Western -blot, respectively.Results The EB contents of each time point groups were higher than sham operation group (P<0.01).The expression of ZO-1 in each time point groups were lower than sham operation group (P <0.01).And the level of ZO -1 expression was continuously decreased during the development of sepsis .Conclusion The expression levels of ZO -1 mRNA and protein in sepsis rats are decreased significantly , and this may be one of the mechanisms of blood-brain barrier damage of sepsis .