绥化学院学报
綏化學院學報
수화학원학보
JOURNAL OF SUIHUA TEACHERS COLLEGE
2014年
9期
150-153
,共4页
陶志杰%王睿%王改玲%许晖%朱晓霞%孙晓侠
陶誌傑%王睿%王改玲%許暉%硃曉霞%孫曉俠
도지걸%왕예%왕개령%허휘%주효하%손효협
花椒%油树脂%纤维素酶%正交试验
花椒%油樹脂%纖維素酶%正交試驗
화초%유수지%섬유소매%정교시험
Zanthoxylum bungeanum%Oleoresin%Cellulase%Orthogonal experiment
试验以纤维素酶法辅助优化提取花椒油树脂。通过利用单因素和正交试验分析,结果表明,酶解温度为影响提取效果的显著因子,优化后提取条件为酶用量2.0mg/100mL,酶解时间1.5h,酶解温度45℃,在该工艺条件下提取一次花椒油树脂的平均提取率为30.2%。验证试验表明,经酶解提取法提取花椒油树脂与未经酶解处理的乙醇浸提法相比,结果增长25.8%。
試驗以纖維素酶法輔助優化提取花椒油樹脂。通過利用單因素和正交試驗分析,結果錶明,酶解溫度為影響提取效果的顯著因子,優化後提取條件為酶用量2.0mg/100mL,酶解時間1.5h,酶解溫度45℃,在該工藝條件下提取一次花椒油樹脂的平均提取率為30.2%。驗證試驗錶明,經酶解提取法提取花椒油樹脂與未經酶解處理的乙醇浸提法相比,結果增長25.8%。
시험이섬유소매법보조우화제취화초유수지。통과이용단인소화정교시험분석,결과표명,매해온도위영향제취효과적현저인자,우화후제취조건위매용량2.0mg/100mL,매해시간1.5h,매해온도45℃,재해공예조건하제취일차화초유수지적평균제취솔위30.2%。험증시험표명,경매해제취법제취화초유수지여미경매해처리적을순침제법상비,결과증장25.8%。
The present study was conducted to optimize the extraction of oleoresin from Zanthoxylum bungeanum with cellulase. By using one-factor-at-a-time and orthogonal array design methods, the results showed that hydrolysis temperature was the significant factor, enzyme dose of 2.0 mg/100 mL, hydrolysis time of 1.5 h and hydrolysis temperature of 45℃. Under the optimum conditions, the extraction efficiency of oleoresin was 30.2%, 25.8%higher than the yield of ethanol extraction.