CAJ | 학술논문

目的：评价玻璃体腔内注射大麻素 HU-211对大鼠青光眼模型视神经的保护作用,为青光眼视神经损伤治疗提供实验依据。 　　方法：采用电凝巩膜表面静脉法制作大鼠青光眼模型18眼,随机分为3组：A 组分别隔日一次玻璃体腔注射1mg/0.1mL大麻素 HU-211,B 组隔日一次玻璃体腔注射0.1mL 生理盐水,C 组为高眼压组。随机选6只对侧眼为空白对照组,每日观察眼压变化情况,用药4wk 后处死大鼠,视网膜冰冻切片,HE 染色通过视网膜神经元的密度变化评估大鼠慢性高眼压模型视网膜神经元的损伤程度。 　　结果： B 组的凋亡程度及 RGC 的损伤程度明显高于 A组,差异有统计学意义( P<0.05), B 组与 C 组比较差异无统计学意义(P>0.05)。 　　结论：玻璃体腔注射大麻素(HU-211)对大鼠青光眼模型视神经视网膜有明显的保护作用。
목적：평개파리체강내주사대마소 HU-211대대서청광안모형시신경적보호작용,위청광안시신경손상치료제공실험의거。 　　방법：채용전응공막표면정맥법제작대서청광안모형18안,수궤분위3조：A 조분별격일일차파리체강주사1mg/0.1mL대마소 HU-211,B 조격일일차파리체강주사0.1mL 생리염수,C 조위고안압조。수궤선6지대측안위공백대조조,매일관찰안압변화정황,용약4wk 후처사대서,시망막빙동절편,HE 염색통과시망막신경원적밀도변화평고대서만성고안압모형시망막신경원적손상정도。 　　결과： B 조적조망정도급 RGC 적손상정도명현고우 A조,차이유통계학의의( P<0.05), B 조여 C 조비교차이무통계학의의(P>0.05)。 　　결론：파리체강주사대마소(HU-211)대대서청광안모형시신경시망막유명현적보호작용。
To evaluate the protective effects of intravitreal injection of cannabinoid HU - 211 for optic nerve in glaucoma rats, providing the experimental evidence in the treatment of glaucoma optic nerve injury. ●METHODS: Glaucoma rats (18 eyes) were modeled by electric coagulation sclera surface vein and randomized into 3 groups, group A received intravitreal injection of 1mg/ 0. 1mL cannabinoid HU - 211 every other day respectively; group B was given intravitreal injection of 0. 1mL water every other day, group C was high intraocular pressure (lOP) group, 6 eyes were randomly selected for blank control group ( group D). lOP was observed every day. The rats were sacrificed after treatment 4wk, froze retina section, HE stain. The density fluctuation of retinal ganglion cell ( RGC ) neurons assessment the optic nerve of rat model with chronic high lOP glaucoma were measured. ●RESULTS: The apoptosis and damage degree of RGC in group B was obviously higher than that in group A, with statistically significant difference (P<0. 05). There was no significant difference in groups B and C (P >0. 05). ●CONCLUSlON: lntravitreal injection of cannabinoid HU-211 shows obvious protective effect on optic nerve in glaucom rat models.