CAJ | 학술논문

目的：探讨地塞米松对缝线诱导的兔角膜新生血管组织中 IL-1β及 TNF-α表达的影响,分析地塞米松治疗角膜新生血管( corneal neovascularization, CNV)可能的分子机制。 　　方法：成年家兔43只,随机选取40只兔双眼行角膜基质层缝线建立兔角膜新生血管模型,造模成功后右眼( A组)未行特殊处理,左眼(B 组)给予地塞米松局部注射,未造模3只兔双眼为正常对照,造模后裂隙灯动态观察新生血管的形态并计算其生长面积,并分别于造模后1,4,7,14,21d 各处死8只兔,取角膜新生血管组织行 HE染色观察其病理学特点,并检测角膜中 IL-1β及 TNF-α的表达情况。 　　结果：缝线后4d 可见 CNV 长入,7~14d 生长最为旺盛, HE 染色见炎性细胞浸润,浅基质层大量新生血管生长,免疫组化染色提示,随缝线时间延长角膜 IL-1β及 TNF-α的表达逐渐增加；而经地塞米松治疗后,CNV 较前延迟长入,面积缩小,炎性细胞浸润减轻,且角膜中 IL-1β及TNF-α的表达较单纯缝线眼明显降低,结果有统计学意义(P<0.05)。 　　结论：地塞米松可能通过早期抑制角膜组织中 IL-1β及TNF-α表达而抑制新生血管的生长。
목적：탐토지새미송대봉선유도적토각막신생혈관조직중 IL-1β급 TNF-α표체적영향,분석지새미송치료각막신생혈관( corneal neovascularization, CNV)가능적분자궤제。 　　방법：성년가토43지,수궤선취40지토쌍안행각막기질층봉선건립토각막신생혈관모형,조모성공후우안( A조)미행특수처리,좌안(B 조)급여지새미송국부주사,미조모3지토쌍안위정상대조,조모후렬극등동태관찰신생혈관적형태병계산기생장면적,병분별우조모후1,4,7,14,21d 각처사8지토,취각막신생혈관조직행 HE염색관찰기병이학특점,병검측각막중 IL-1β급 TNF-α적표체정황。 　　결과：봉선후4d 가견 CNV 장입,7~14d 생장최위왕성, HE 염색견염성세포침윤,천기질층대량신생혈관생장,면역조화염색제시,수봉선시간연장각막 IL-1β급 TNF-α적표체축점증가；이경지새미송치료후,CNV 교전연지장입,면적축소,염성세포침윤감경,차각막중 IL-1β급TNF-α적표체교단순봉선안명현강저,결과유통계학의의(P<0.05)。 　　결론：지새미송가능통과조기억제각막조직중 IL-1β급TNF-α표체이억제신생혈관적생장。
To discuss the influence of dexamethasion on lL-1β and TNF - α expression in suture - induced rabbit corneal neovascularization ( CNV ) and analyze the potential mechanism. ●METHODS: For 43 healthy rabbits, 40 were randomly selected for establishing CNV model in corneal stroma. The right eyes (group A) were received no medicine and the left eyes ( group B) were injected dexamethasone after successfully establishing the model. The no modeling 3 rabbits were normal control group. The morphologic change of corneal was observed with slit lamp microscope and the areas of CNV was calculated every day, then 8 rabbits were randomly chosen for sacrificing at 1, 4, 7, 14, 21d respectively. The pathological characteristics of CNV were observed after HE staining, and lL - 1β and TNF - α expression was detected by immunohistochemistry. ●RESULTS: CNV was grown at the 4d after suture, and the 7-14d was vigorous growth period. inflammatory cell infiltration appeared after HE staining, and CNV was located at the superficial stroma of cornea. lmmunohistochemistry results showed that lL - 1β and TNF - α expression was gradually increased with prolonged suture time. Compared with corneal stitch group, the rabbits cured by dexamethasone were found with less inflammatory cells infiltrating and neovescularization, moreover, the expression of lL - 1βand TNF-α decreased. There were statistical significance between the two groups (P<0. 05). ● CONCLUSlON: Dexamethasone can inhibit the CNV growth by controlling the inflammation of corneal and restraining lL-1β and TNF-α expression.