CAJ | 학술논문

目的：探讨银杏叶提取物对内皮细胞凋亡的影响及其作用机制。方法将孵育好的人脐静脉内皮细胞分成5组：内皮细胞组（对照组）；内皮细胞＋游离脂肪酸（FFA）组（实验1组）；内皮细胞＋FFA＋10μl 银杏叶提取物0．35 mg/L 组（实验2组）；内皮细胞＋FFA＋10μl 银杏叶提取物3．5 mg/L 组（实验3组）；内皮细胞＋FFA＋10μl 银杏叶提取物35 mg/L 组（实验4组）。分别测定丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX-1）以及细胞凋亡率。结果与对照组比较，实验1组内皮细胞上清液中 SOD 和 GPX-1含量显著降低（P ＜0．01），而MDA 则显著升高（P ＜0．01）；与实验1组比较，实验2、3、4组内皮细胞上清液中 SOD 和 GPX-1含量显著升高（P ＜0．01），MDA 显著降低（P ＜0．01）。实验1组 FFA 诱导的内皮细胞凋亡率显著高于对照组（P ＜0．01）；经银杏叶提取物注射后，实验2、3、4组内皮细胞凋亡率显著低于实验1组（P ＜0．01）。结论银杏叶提取物可通过改善血管内皮细胞的氧化应激水平而抑制其凋亡。
목적：탐토은행협제취물대내피세포조망적영향급기작용궤제。방법장부육호적인제정맥내피세포분성5조：내피세포조（대조조）；내피세포＋유리지방산（FFA）조（실험1조）；내피세포＋FFA＋10μl 은행협제취물0．35 mg/L 조（실험2조）；내피세포＋FFA＋10μl 은행협제취물3．5 mg/L 조（실험3조）；내피세포＋FFA＋10μl 은행협제취물35 mg/L 조（실험4조）。분별측정병이철（MDA）、초양화물기화매（SOD）、곡광감태과양화물매（GPX-1）이급세포조망솔。결과여대조조비교，실험1조내피세포상청액중 SOD 화 GPX-1함량현저강저（P ＜0．01），이MDA 칙현저승고（P ＜0．01）；여실험1조비교，실험2、3、4조내피세포상청액중 SOD 화 GPX-1함량현저승고（P ＜0．01），MDA 현저강저（P ＜0．01）。실험1조 FFA 유도적내피세포조망솔현저고우대조조（P ＜0．01）；경은행협제취물주사후，실험2、3、4조내피세포조망솔현저저우실험1조（P ＜0．01）。결론은행협제취물가통과개선혈관내피세포적양화응격수평이억제기조망。
Objective To study the effect of Ginkgo biloba extract on apoptosis of endothelial cells and its mechanism.Methods The incubated human umbilical vein endothelial cells fell into five groups:endothelial cell group (the control group);endothelial cell+ FFA group (the experimental group 1);endothelial cell+ FFA+ 10 μl Ginkgo biloba extract 0.35 mg/L group(the experimental group 2);endothelial cell+ FFA+ 10 μl Ginkgo biloba extract 3.5 mg/L group(the experimental group 3);endothelial cell+ FFA+ 10 μl Ginkgo biloba extract 35 mg/L group(the experimental group 4).Superoxide dismutase(SOD),glutathione peroxidase(GPX-1),malondialdehyde(MDA),and rate of cell apoptosis were respectively measured.Results Compared with the control group,SOD and GPX-1 in endothelial cell supernatant of the experimental group 1 significantly decreased(P < 0.01),MDA significantly increased (P <0.01).Compared with the experimental 1,SOD and GPX-1 in endothelial cell supernatant of the experimental group 2, 3,4 significantly decreased (P < 0.01 ),but MDA increased significantly (P < 0.01 ).FFA-induced endothelial cell apoptosis of the experimental group 1 was significantly higher than that of the control group(P <0.01);After the injection of Ginkgo biloba extract intervention,endothelial cell apoptosis of the experimental group 2,3,4 was significantly lower than that of the experimental group (P <0.01).Conclusion Ginkgo biloba extract can improve the level of oxidative stress in vascular endothelial cells and inhibit the apoptosis.