化工进展
化工進展
화공진전
CHEMICAL INDUSTRY AND ENGINEERING PROGRESS
2014年
9期
2425-2431
,共7页
沈萨萨%姜灵%陆杰%于洪巍
瀋薩薩%薑靈%陸傑%于洪巍
침살살%강령%륙걸%우홍외
酶法动态动力学拆分%脂肪酶Pseudomonas sp. ECU1011%扁桃酸消旋酶%乙酰基邻氯扁桃酸%邻氯扁桃酸
酶法動態動力學拆分%脂肪酶Pseudomonas sp. ECU1011%扁桃痠消鏇酶%乙酰基鄰氯扁桃痠%鄰氯扁桃痠
매법동태동역학탁분%지방매Pseudomonas sp. ECU1011%편도산소선매%을선기린록편도산%린록편도산
enzymatic dynamic kinetic resolution%Pseudomonas sp. ECU1011 lipase%mandelate racemase%R-(-)-acetyl-o-mandelic acid%chloromandelic acid
采用假单胞菌脂肪酶Pseudomonas sp. ECU1011催化乙酰基邻氯扁桃酸进行不对称水解,利用突变后的扁桃酸消旋酶(V29I)对拆分后的产物 S-(-)-邻氯扁桃酸进行消旋,消旋后的邻氯扁桃酸经过酰化重新被利用到水解反应中,实现了酶法动态动力学拆分制备R-(-)-乙酰基邻氯扁桃酸。通过对拆分反应、拆分混合物的分离回收以及消旋反应的工艺优化,最终获得光学纯度ee>99.9%的R-(-)-乙酰基邻氯扁桃酸,其收率达80%。本研究建立的R-(-)-乙酰基邻氯扁桃酸的动态动力学拆分工艺,对其工业化应用具有重要的指导意义。
採用假單胞菌脂肪酶Pseudomonas sp. ECU1011催化乙酰基鄰氯扁桃痠進行不對稱水解,利用突變後的扁桃痠消鏇酶(V29I)對拆分後的產物 S-(-)-鄰氯扁桃痠進行消鏇,消鏇後的鄰氯扁桃痠經過酰化重新被利用到水解反應中,實現瞭酶法動態動力學拆分製備R-(-)-乙酰基鄰氯扁桃痠。通過對拆分反應、拆分混閤物的分離迴收以及消鏇反應的工藝優化,最終穫得光學純度ee>99.9%的R-(-)-乙酰基鄰氯扁桃痠,其收率達80%。本研究建立的R-(-)-乙酰基鄰氯扁桃痠的動態動力學拆分工藝,對其工業化應用具有重要的指導意義。
채용가단포균지방매Pseudomonas sp. ECU1011최화을선기린록편도산진행불대칭수해,이용돌변후적편도산소선매(V29I)대탁분후적산물 S-(-)-린록편도산진행소선,소선후적린록편도산경과선화중신피이용도수해반응중,실현료매법동태동역학탁분제비R-(-)-을선기린록편도산。통과대탁분반응、탁분혼합물적분리회수이급소선반응적공예우화,최종획득광학순도ee>99.9%적R-(-)-을선기린록편도산,기수솔체80%。본연구건립적R-(-)-을선기린록편도산적동태동역학탁분공예,대기공업화응용구유중요적지도의의。
In this study,asymmetric hydrolysis of acetyl chloromandelic acid was catalyzed by Pseudomonas sp. ECU1011,and the S-(-)-chloromandelic acid in the resolution mixture was then racemerized using the mutated mandelate racemase and fed back into the hydrolysis reaction after acylation , realizing enzymatic dynamic kinetic resolution for preparation of R-(-)-acetyl chloromandelic acid. Firstly,the conditions for enzymatic resolution,separation of resolution mixture, and enzymatic racemization were optimized. Subsequently,dynamic kinetic resolution of acetyl chloromandelic acid was conducted under the optimal conditions,obtaining optically pure R-(-)-acetyl chloromandelic acid with final yield of 80%. The conditions for enzymatic resolution,chloromandelic acid recycling and racemization were optimized in this study,leading to preliminary establishment of the dynamic kinetic resolution process for R-(-)-acetyl chloromandelic acid preparation,with guiding significance for future industrial applications.