CAJ | 학술논문

为探讨蛹虫草表型特征与其发酵产虫草菌素间的关系，以采自于3个不同地区的野生蛹虫草为供试菌株，对其不同单子囊孢子菌株进行分型，并研究表型多态性对蛹虫草发酵产虫草素的影响。结果表明：1）绝大多数单子囊孢子菌落生长速率正常，大部分的菌落颜色为杏橙色，其次分别为橙铬色、淡柠檬色或者白色。2）采自于贵州六盘水玉舍国家森林公园的08Y1菌株虫草素产量较高，达（125．52±5．57）mg/瓶；菌落背面颜色为橙铬色时虫草素产量较高，为（141．34±5．41）mg/瓶。3）多数出发菌株（原代）虫草素产量高于其角变株，但也有少数的角变株相对于其出发菌株虫草素产量有明显的增加，最大提高2．87倍，并且同一出发菌株的不同角变株虫草素产量可能升高或降低。因此，分离角变株可成为筛选蛹虫草虫草素高产优势菌株的新方法。
위탐토용충초표형특정여기발효산충초균소간적관계，이채자우3개불동지구적야생용충초위공시균주，대기불동단자낭포자균주진행분형，병연구표형다태성대용충초발효산충초소적영향。결과표명：1）절대다수단자낭포자균락생장속솔정상，대부분적균락안색위행등색，기차분별위등락색、담저몽색혹자백색。2）채자우귀주륙반수옥사국가삼림공완적08Y1균주충초소산량교고，체（125．52±5．57）mg/병；균락배면안색위등락색시충초소산량교고，위（141．34±5．41）mg/병。3）다수출발균주（원대）충초소산량고우기각변주，단야유소수적각변주상대우기출발균주충초소산량유명현적증가，최대제고2．87배，병차동일출발균주적불동각변주충초소산량가능승고혹강저。인차，분리각변주가성위사선용충초충초소고산우세균주적신방법。
The wild C.militaris ascospore strains from three different areas were analyzed to study the effect of phenotypic polymorphism on cordycepin yield and discuss the relationship between C.militaris phenotypic characteristics and cordycepin yield.The results showed that:1 )The colony color of most ascospores with normal growth rate is mainly apricot orange,followed by orange chrome,shallow lemon or white;2)The cordycepin yield of 08Y1 strain from Guizhou Yushe National Forest Park is 125.52 5.57 mg/bottle and cordycepin yield of 08Y1 strain with orange chrome color can reach 141.34 5.41 mg/bottle;3)The cordycepin yield of most original strains is higher than its sector mutation strains but the cordycepin yield of the minority sector mutation strains can be increased by 2.87 times compared with their original strains.Therefore, the isolated sector mutation strains may be a new method to select dominant C.militaris strains with high cordycepin yield.