CAJ | 학술논문

为筛选出有效防治杜仲灰斑病的药剂在生产上应用，采用菌丝生长速率法，研究甲基托布津、百菌清、中生菌素、多抗霉素、多菌灵和苯醚甲环唑等6种杀菌剂对杜仲灰斑病菌的室内毒力，并进行了田间药效试验。结果表明：室内毒力测定，多抗霉素、多菌灵和苯醚甲环唑对灰斑病菌有较好的抑制作用，其EC50分别为1．67μg/mL、0．96μg/mL和0．18μg/mL；而甲基托布津、百菌清和中生菌素对灰斑病菌的毒力相对较弱。经田间药效试验，多抗霉素1000倍液、多菌灵2000倍液和苯醚甲环唑2500倍液对杜仲灰斑病有较好的防治效果，第2次施药10 d后的防效分别可达74．36％、80．82％和79．67％，显著优于其他药剂处理，可作为防治杜仲灰斑病的首选药剂。
위사선출유효방치두중회반병적약제재생산상응용，채용균사생장속솔법，연구갑기탁포진、백균청、중생균소、다항매소、다균령화분미갑배서등6충살균제대두중회반병균적실내독력，병진행료전간약효시험。결과표명：실내독력측정，다항매소、다균령화분미갑배서대회반병균유교호적억제작용，기EC50분별위1．67μg/mL、0．96μg/mL화0．18μg/mL；이갑기탁포진、백균청화중생균소대회반병균적독력상대교약。경전간약효시험，다항매소1000배액、다균령2000배액화분미갑배서2500배액대두중회반병유교호적방치효과，제2차시약10 d후적방효분별가체74．36％、80．82％화79．67％，현저우우기타약제처리，가작위방치두중회반병적수선약제。
In order to screen effective fungicides controlling gray leaf spot disease of E.ulmoides,the toxicity of six fungicides(thiophanate methyl,chlorothalonil,Zhongshengmycin,polyoxins,carbendazim and difenoconazole)on E.ulmoides gray leaf spot pathogen were determined in laboratory using mycelium growth rate method.The field trial of the six fungicides controlling gray leaf spot disease of E.ulmoides was also carried out under the field condition. Results:Thiophanate-methyl, chlorothalonil and zhongshengmycin had worse antimicrobial effect on A.tenuis.polyoxin,carbendazim and difenoconazole had better antimicrobial effect on A.tenuis than others,with EC50 of 1.67μg/mL,0.96μg/mL and 0.18μg/mL,respectively.The field trial results also confirmed that ten days after second sprayed application, the control efficiency of polyoxin 1 000 times,carbendazim 2 000 times and difenoconazole 2 500 times reached 74.36%, 80.82% and 79.67%, respectively. Control eficiency of the three agents was significantly better than that of other treatments.Therefore,polyoxin,carbendazim and difenoconazole could be recommended as the first-choice fungicides to control gray leaf spot disease of E.ulmoides.