CAJ | 학술논문

为获得小麦中的DREB转录因子基因，运用电子克隆的方法，以拟南芥NP_195489序列为探针，获得小麦DREB的 cDNA全长序列。采用生物信息学方法，对该基因编码蛋白的基本特征、高级结构及功能域等进行预测和分析。结果表明：该基因全长2008 bp，包含1个639 bp的完整开放性阅读框，编码212个氨基酸，该基因编码蛋白定位于细胞核，为可溶性蛋白，含有多个 AP2保守功能域。在小麦幼穗、幼苗、花药、种子、根、叶片和花冠中为组成型表达，其中在根和叶片中的表达量比其他组织类型中表达量高。
위획득소맥중적DREB전록인자기인，운용전자극륭적방법，이의남개NP_195489서렬위탐침，획득소맥DREB적 cDNA전장서렬。채용생물신식학방법，대해기인편마단백적기본특정、고급결구급공능역등진행예측화분석。결과표명：해기인전장2008 bp，포함1개639 bp적완정개방성열독광，편마212개안기산，해기인편마단백정위우세포핵，위가용성단백，함유다개 AP2보수공능역。재소맥유수、유묘、화약、충자、근、협편화화관중위조성형표체，기중재근화협편중적표체량비기타조직류형중표체량고。
The full-length cDNA sequence of DREB gene was obtained by in silico cloning using NP_195489 sequence from Arabidopsis as the probe sequence.Some characters of the clone gene encoding amino acid,including the composition of amino acid sequence,physical and chemical properties,secondary and tertiary structure of protein plus functional domains,were analyzed by bioinformatics tools.The results showed that the full-length gene from wheat was 2008 bp,including one 639bp open reading frame (ORF) which encodes a polypeptide of 212 amino acids.The encoded protein of the clone gene,with several conserved AP2 domain sequences, was soluble and located in nucleus. Electronic expression analysis revealed that the clone gene was constitutively expressed in the wheat young ear,seedling,semet,root, leaf and corol,and the expression of this gene in the root and leaf of wheat was much higher than that in all the other types of wheat tissues.