CAJ | 학술논문

采用傅里叶变换红外光谱对杜鹃花科植物闹羊花与菊科植物野菊花两种花类中药材的主成分进行表征，其中闹羊花出现在1648和1543cm-1出现明显的酰胺Ⅰ带和酰胺Ⅱ带吸收峰，证明其中含有蛋白质，可能与其具有的免疫作用相关；野菊花出现在1734和1515cm-1等CO伸缩振动吸收峰，证明其中含有挥发油等酯类成分。由于二者的红外光谱具有较高的相似性且难以区分，通过二级鉴别比较闹羊花与野菊花的二阶导数红外光谱可看出，在1656和1515cm-1附近的吸收峰，表明二者均含有黄酮类成分；野菊花的二阶导数谱图中出现在1163，1077，1026，986和869cm-1的吸收峰与菊糖的特征吸收峰一致，因此可以看出野菊花中含有菊糖。选取不同波数范围的闹羊花与野菊花的二维相关红外光谱进行对比，发现在1700～1400cm-1黄酮类成分区域闹羊花有3个明显的自动峰，野菊花有10个明显的自动峰；在1250～900cm-1糖苷类成分区域闹羊花有10个明显的自动峰，野菊花有9个明显的自动峰。红外光谱的“三级鉴定法”为中药材的鉴别提供更快速、准确、方便的手段。
채용부리협변환홍외광보대두견화과식물료양화여국과식물야국화량충화류중약재적주성분진행표정，기중료양화출현재1648화1543cm-1출현명현적선알Ⅰ대화선알Ⅱ대흡수봉，증명기중함유단백질，가능여기구유적면역작용상관；야국화출현재1734화1515cm-1등CO신축진동흡수봉，증명기중함유휘발유등지류성분。유우이자적홍외광보구유교고적상사성차난이구분，통과이급감별비교료양화여야국화적이계도수홍외광보가간출，재1656화1515cm-1부근적흡수봉，표명이자균함유황동류성분；야국화적이계도수보도중출현재1163，1077，1026，986화869cm-1적흡수봉여국당적특정흡수봉일치，인차가이간출야국화중함유국당。선취불동파수범위적료양화여야국화적이유상관홍외광보진행대비，발현재1700～1400cm-1황동류성분구역료양화유3개명현적자동봉，야국화유10개명현적자동봉；재1250～900cm-1당감류성분구역료양화유10개명현적자동봉，야국화유9개명현적자동봉。홍외광보적“삼급감정법”위중약재적감별제공경쾌속、준학、방편적수단。
In this study ,major chemical components of Flos rhododendri mollis and Flos chrysanthemi indici were characterized using Fourier transform infrared spectroscopy (FTIR) .For Flos rhododendri mollis ,the bands at 1648 and 1543 cm -1were at-tributed to amide Ⅰ and amide Ⅱ ,respectively ,indicating that it contained proteins probably resulting in immunization .In case of Flos chrysanthemi indici ,stretching vibration of CO function group was responsible for the bands at 1734 and 1515 cm -1,as a result of essential oils ,lipids ,etc .Since FTIR spectra of Flos rhododendri mollis and Flos chrysanthemi indici are almost identical and it is difficult to discriminate them ,two-step identification was investigated via secondary derivative of the FTIR spectra .The bands at 1656 and 1515 cm-1corresponds to flavonoides in Flos rhododendri mollis and Flos chrysanthemi indici .In the secondary derivative of the FTIR spectrum of Flos chrysanthemi indici ,characteristic bands of inulin were present at 1163 ,1077 ,1026 ,986 and 869 cm -1,and therefore Flos chrysanthemi indici contained inulin as well .Tri-step identifica-tion was carried out for Flos rhododendri mollis and Flos chrysanthemi indici by means of comparing their 2D-IR correlation spectra in different wave number range .In the characteristic range of flavonoides (1700~1400 cm -1) ,Flos rhododendri mollis exhibited 3 obvious autopeaks ,while 10 autopeaks were visualized in the 2D-IR correlation spectrum of Flos chrysanthemi indici Moreover ,in the characteristic range of glucoside (1250~900 cm-1) ,10 and 9 autopeaks were present in the 2D-IR correlation spectra of Flos rhododendri mollis and Flos chrysanthemi indici ,respectively .Therefore ,the tri-step identification of FTIR is a time-saving ,accurate ,cost-saving and convenient method to effectively distinguish traditional Chinese medicines .