CAJ | 학술논문

利用共有峰率和变异峰率双指标，以胡椒属七种药用植物的红外指纹图谱为依据，计算出所测样品的共有峰率和变异峰率。建立了胡椒属七种药用植物共有峰率和变异峰率双指标序列，结果揭示了胡椒属七种药用植物的亲缘关系。中药海风藤与石南藤、大叶蒟根茎的共有峰率均在77％以上，变异峰率均在30％以下，此三种胡椒属药用植物亲缘关系较近；海风藤与胡椒根茎、十八症根的共有峰率在61％左右，亲缘关系稍远；海风藤与蒌叶根茎的共有峰率仅为44％。亲缘关系较远。利用红外二阶导数谱图，可以鉴别中药海风藤及其伪品石南藤、十八症根、大叶蒟根茎、胡椒根茎等品种。傅里叶红外光谱法可以对样品进行无损处理，不需要对药材进行复杂的提取分离过程即可提供化合物的官能团、类别、氢键等信息。该法能从整体水平分析谱图特征峰，具有分析速度快，重现性好，非破坏性和样品量小，制样简单，专属性强，节约成本，保护环境等优点。可以解决中药海风藤因资源短缺，伪品较多，鉴别困难等问题，也可为临床用药的安全、有效提供保障。傅里叶红外光谱法为胡椒属药用植物亲缘关系的探讨和真伪鉴别提供了一种新方法。符合中药向综合分析和整体分析发展的趋势。
이용공유봉솔화변이봉솔쌍지표，이호초속칠충약용식물적홍외지문도보위의거，계산출소측양품적공유봉솔화변이봉솔。건립료호초속칠충약용식물공유봉솔화변이봉솔쌍지표서렬，결과게시료호초속칠충약용식물적친연관계。중약해풍등여석남등、대협구근경적공유봉솔균재77％이상，변이봉솔균재30％이하，차삼충호초속약용식물친연관계교근；해풍등여호초근경、십팔증근적공유봉솔재61％좌우，친연관계초원；해풍등여루협근경적공유봉솔부위44％。친연관계교원。이용홍외이계도수보도，가이감별중약해풍등급기위품석남등、십팔증근、대협구근경、호초근경등품충。부리협홍외광보법가이대양품진행무손처리，불수요대약재진행복잡적제취분리과정즉가제공화합물적관능단、유별、경건등신식。해법능종정체수평분석보도특정봉，구유분석속도쾌，중현성호，비파배성화양품량소，제양간단，전속성강，절약성본，보호배경등우점。가이해결중약해풍등인자원단결，위품교다，감별곤난등문제，야가위림상용약적안전、유효제공보장。부리협홍외광보법위호초속약용식물친연관계적탐토화진위감별제공료일충신방법。부합중약향종합분석화정체분석발전적추세。
The common peak ratio and variant peak ratio were calculated by FTIR spectroscopy of seven medicinal plants of Pip-er .The dual index sequence of common peak ratio and variant peak ratio was established ,which showed the sibship of the medic-inal plants .The common peak ratio of Piper kadsura (Choisy) Ohwi ,Piper wallichii (Miq .) Hand .-Mazz .Piper laetispicum (C .DC .) was greater than 77% ,and the variant peak ratio was less than 30% .The results showed the near sibship between the three drugs .The common peak ratio of Piper kadsura (Choisy) Ohwi ,Piper nigrum L .and Piper boehmeriaefolium Wall (Miq .) C .DC .Var .tonkinense (C .DC .) was about 61% which showed the farther sibship .The common peak ratio of Piper kadsura (Choisy) Ohwi and Piper betle (Linn .) was only 44% ,which showed the farthest sibship . Piper kadsura (choisy) Ohwi and its adulterants ,such as Piper wallichii(Miq .) Hand .-Mazz ., Piper boehmeriae folium Wall(Miq .) C .DC .Var . tonkinense C .DC .,Piper laetispicum C .DC .,Piper nigrum L .,could be identified by comparing their second order derivative IR spectrum of the samples .FTIR technique is a non-destructive analysis method which provides information of functional group ,type and hydrogen bond without complex pretreatment procedures such as extraction and separation .FTIR method has some characteristics such as rapid and simple analysis procedure ,good reproducibility ,non-destructive testing ,few amount of required sample and low cost and is environment-friendly .The method solved the problems of limit in resource of Piper kadsura (Choisy) Ohwi ,many fakes and difficulties in identification ,and brought the security for the clinical medication .FTIR provides a new method for identification of Piper kadsura(choisy) Ohwi and its fakes and meets the requirement for comprehensive analy-sis and global analysis of traditional Chinese medicine .