CAJ | 학술논문

以二氧化硅-氧化石墨烯复合物为固相萃取材料,建立了植物油中黄曲霉毒素B1、B2的高效液相色谱(HPLC)检测方法。优化的条件为：复合材料的最佳用量为0.15 g,最佳萃取时间20 min,洗脱溶剂为乙腈,洗脱次数为2次。结果表明,在优化条件下,建立的二氧化硅-氧化石墨烯复合物固相萃取-高效液相色谱法对黄曲霉毒素B1、B2的检出限分别为0.17和0.05μg/L。将本方法应用于植物油实际样品的检测中,加标回收率在81.4%~105.3%之间,相对标准偏差为1.3%~8.6%。
이이양화규-양화석묵희복합물위고상췌취재료,건립료식물유중황곡매독소B1、B2적고효액상색보(HPLC)검측방법。우화적조건위：복합재료적최가용량위0.15 g,최가췌취시간20 min,세탈용제위을정,세탈차수위2차。결과표명,재우화조건하,건립적이양화규-양화석묵희복합물고상췌취-고효액상색보법대황곡매독소B1、B2적검출한분별위0.17화0.05μg/L。장본방법응용우식물유실제양품적검측중,가표회수솔재81.4%~105.3%지간,상대표준편차위1.3%~8.6%。
Silica dioxide bound graphene oxide ( GO-SiO2 ) was applied as an effective adsorbent for determination and quantiflcation of aflatoxin B1 , B2 in edible oil by HPLC. The optimized conditions were GO-SiO2 0. 15 g, extraction time 20 min, elution reagent acetonitrile, elution cycles two times. Results showed under the optimum conditions, the detection limits of aflatoxin B1 , B2 were 0. 17 and 0. 05 μg/L, respectively. The method was successfully applied to the detection of the actual edible oil, the spiked recoveries of aflatoxin B1 and B2 were 81. 4%-105. 3% and the relative standard deviations were 1. 3%-8. 6%.