分析化学
分析化學
분석화학
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2014年
9期
1332-1337
,共6页
马晓玲%丁承君%张璞%郭玮%罗红霞
馬曉玲%丁承君%張璞%郭瑋%囉紅霞
마효령%정승군%장박%곽위%라홍하
季铵盐化纳米金刚石%肌红蛋白%化学修饰电极%循环伏安法%过氧化氢
季銨鹽化納米金剛石%肌紅蛋白%化學脩飾電極%循環伏安法%過氧化氫
계안염화납미금강석%기홍단백%화학수식전겁%순배복안법%과양화경
Quaternarized nanodiamond%Myoglobin%Chemically modified electrode%Cyclic voltammetry%Hydrogen peroxide
通过一系列的化学反应对纳米金刚石( ND )表面进行修饰,成功制备了季铵盐化纳米金刚石:ND-CO-NH-CH2-CH2-N( CH3)3+·I-( QAS-ND),通过FT-IR、元素分析、电化学等手段对目标产物QAS-ND进行了表征。将肌红蛋白( Mb)与QAS-ND混合液滴加在玻碳( GC)电极表面,制备QAS-ND/Mb/GC修饰电极。在0.1 mol/L磷酸盐缓冲溶液(pH 7.0)中,固定在膜内的Mb表现出良好的直接电化学性质,并显示了很好的稳定性。同时,探讨了此修饰电极表面固定的Mb对H2 O2的催化还原,结果表明,此修饰电极可作为H2 O2生物传感器,实现对H2O2的快速、准确检测,检出限为3.5μmol/L (S/N=3)。
通過一繫列的化學反應對納米金剛石( ND )錶麵進行脩飾,成功製備瞭季銨鹽化納米金剛石:ND-CO-NH-CH2-CH2-N( CH3)3+·I-( QAS-ND),通過FT-IR、元素分析、電化學等手段對目標產物QAS-ND進行瞭錶徵。將肌紅蛋白( Mb)與QAS-ND混閤液滴加在玻碳( GC)電極錶麵,製備QAS-ND/Mb/GC脩飾電極。在0.1 mol/L燐痠鹽緩遲溶液(pH 7.0)中,固定在膜內的Mb錶現齣良好的直接電化學性質,併顯示瞭很好的穩定性。同時,探討瞭此脩飾電極錶麵固定的Mb對H2 O2的催化還原,結果錶明,此脩飾電極可作為H2 O2生物傳感器,實現對H2O2的快速、準確檢測,檢齣限為3.5μmol/L (S/N=3)。
통과일계렬적화학반응대납미금강석( ND )표면진행수식,성공제비료계안염화납미금강석:ND-CO-NH-CH2-CH2-N( CH3)3+·I-( QAS-ND),통과FT-IR、원소분석、전화학등수단대목표산물QAS-ND진행료표정。장기홍단백( Mb)여QAS-ND혼합액적가재파탄( GC)전겁표면,제비QAS-ND/Mb/GC수식전겁。재0.1 mol/L린산염완충용액(pH 7.0)중,고정재막내적Mb표현출량호적직접전화학성질,병현시료흔호적은정성。동시,탐토료차수식전겁표면고정적Mb대H2 O2적최화환원,결과표명,차수식전겁가작위H2 O2생물전감기,실현대H2O2적쾌속、준학검측,검출한위3.5μmol/L (S/N=3)。
Through a series of chemical reactions, a kind of quaternary ammonium salt derivative of nanodiamond, ND-CO-NH-CH2-CH2-N ( CH3 ) 3+· I-( QAS-ND ) , was obtained , which was confirmed by FTIR, element analysis experiment and the electrochemistry measurements. Mixed myoglobin ( Mb) and QAS-ND solution was dropped on the surface of the glassy carbon ( GC ) electrode to prepare QAS-ND/Mb/GC modified electrode. In 0. 1 mol/L phosphate buffer solution (PBS) (pH 7. 0), Mb in the membrane exhibited direct electrochemical properties and showed good stability. The electrocatalytic property of the modified electrode toward H2 O2 was investigated, the results showed that the modified electrode could be used as the H2O2 biosensor to achieve fast, accurate detection of H2O2, with a detection limit of 3. 5 mmol/L (S/N=3).