CAJ | 학술논문

通过一系列的化学反应对纳米金刚石( ND )表面进行修饰，成功制备了季铵盐化纳米金刚石：ND-CO-NH-CH2-CH2-N( CH3)3+·I-( QAS-ND)，通过FT-IR、元素分析、电化学等手段对目标产物QAS-ND进行了表征。将肌红蛋白( Mb)与QAS-ND混合液滴加在玻碳( GC)电极表面，制备QAS-ND/Mb/GC修饰电极。在0.1 mol/L磷酸盐缓冲溶液(pH 7.0)中，固定在膜内的Mb表现出良好的直接电化学性质，并显示了很好的稳定性。同时，探讨了此修饰电极表面固定的Mb对H2 O2的催化还原，结果表明，此修饰电极可作为H2 O2生物传感器，实现对H2O2的快速、准确检测，检出限为3.5μmol/L (S/N=3)。
통과일계렬적화학반응대납미금강석( ND )표면진행수식，성공제비료계안염화납미금강석：ND-CO-NH-CH2-CH2-N( CH3)3+·I-( QAS-ND)，통과FT-IR、원소분석、전화학등수단대목표산물QAS-ND진행료표정。장기홍단백( Mb)여QAS-ND혼합액적가재파탄( GC)전겁표면，제비QAS-ND/Mb/GC수식전겁。재0.1 mol/L린산염완충용액(pH 7.0)중，고정재막내적Mb표현출량호적직접전화학성질，병현시료흔호적은정성。동시，탐토료차수식전겁표면고정적Mb대H2 O2적최화환원，결과표명，차수식전겁가작위H2 O2생물전감기，실현대H2O2적쾌속、준학검측，검출한위3.5μmol/L (S/N=3)。
Through a series of chemical reactions, a kind of quaternary ammonium salt derivative of nanodiamond, ND-CO-NH-CH2-CH2-N ( CH3 ) 3+· I-( QAS-ND ) , was obtained , which was confirmed by FTIR, element analysis experiment and the electrochemistry measurements. Mixed myoglobin ( Mb) and QAS-ND solution was dropped on the surface of the glassy carbon ( GC ) electrode to prepare QAS-ND/Mb/GC modified electrode. In 0. 1 mol/L phosphate buffer solution (PBS) (pH 7. 0), Mb in the membrane exhibited direct electrochemical properties and showed good stability. The electrocatalytic property of the modified electrode toward H2 O2 was investigated, the results showed that the modified electrode could be used as the H2O2 biosensor to achieve fast, accurate detection of H2O2, with a detection limit of 3. 5 mmol/L (S/N=3).