CAJ | 학술논문

利用核酸适配体特异的分子识别功能以及特定荧光基团之间的能量转移,建立了一种高灵敏度、高选择性测定雌二醇的光谱方法。研究了缓冲溶液的pH值、组成和浓度、核酸浓度、实验温度及响应时间等因素对检测雌二醇的影响。在最优的实验条件下(50 mmol/L BR缓冲溶液(pH 7.4),1.0×10-7 mol/L核酸,实验温度45℃,响应时间19 min),体系荧光强度的改变值ΔI与雌二醇浓度的对数 lgC呈良好的线性关系(r=0.9953),线性范围为1.0×10-11~5.0×10-9 mol/L,检出限达6.0×10-12 mol/L(S/N=3)。将本方法用于检测人体尿液中雌二醇的含量,雌二醇的加标回收率为94.0%~103.5%。
이용핵산괄배체특이적분자식별공능이급특정형광기단지간적능량전이,건립료일충고령민도、고선택성측정자이순적광보방법。연구료완충용액적pH치、조성화농도、핵산농도、실험온도급향응시간등인소대검측자이순적영향。재최우적실험조건하(50 mmol/L BR완충용액(pH 7.4),1.0×10-7 mol/L핵산,실험온도45℃,향응시간19 min),체계형광강도적개변치ΔI여자이순농도적대수 lgC정량호적선성관계(r=0.9953),선성범위위1.0×10-11~5.0×10-9 mol/L,검출한체6.0×10-12 mol/L(S/N=3)。장본방법용우검측인체뇨액중자이순적함량,자이순적가표회수솔위94.0%~103.5%。
A highly sensitive fluorescence spectroscopic method was established for the selective determination of estradiol, which took advantages of the excellent molecular recognition capability of aptamer and the energy transfer between the specific fluorescent groups. The effects of the pH value, buffer constituent and concentration, the concentration of DNA, the experimental temperature and response time on the detection of estradiol were studied. Under the optimal conditions (50 mmol/L BR buffer solution with pH value at 7. 4, 1. 0×10-7 mol/L for each DNA strand, incubation at 45 ℃, response time 19 min), the change of the fluorescence intensity (ΔI) versus the logarithm of the concentration of estradiol ( lgC) was linear over a concentration range from 1. 0×10-11 mol/L to 5. 0×10-9 mol/L with good linear correlation (r=0. 9953). The limit of detection (LOD) was found to be 6. 0×10-12 mol/L (S/N=3). This method was successfully applied to the detection of estradiol in human urine, with the recovery in the range of 94. 0%-103. 5%. This method showed good precision and accuracy.