CAJ | 학술논문

利用纸基微芯片便捷、直观的优势,采用吩嗪二甲酯硫酸盐( PMS)/氯化硝基四氮唑蓝( NBT)显色体系,借助凝胶成像仪和普通照相机两种成像方式,建立了纸基微孔阵列芯片比色法检测乳酸脱氢酶( LDH)的方法。在最佳实验条件下,显色强度与LDH浓度呈线性相关。采用凝胶成像仪检测时,线性范围为10~150 U/L,检出限(3σ)为9.44 U/L(n=18)。采用照相法获得的线性范围为15~150 U/L,检出限(3σ)为12.36 U/L(n=18)。实验表明,人血清白蛋白(HSA)对显色结果具有增强作用,探讨了HSA的增色作用,并以HSA为增强试剂得到工作曲线。基于纸基微孔阵列芯片的LDH活性测定方法具有操作简单、结果直观可见、灵敏度高等优点,对于脱氢酶类的便捷检测有一定参考价值,可望在生物医疗检测领域获得应用。
이용지기미심편편첩、직관적우세,채용분진이갑지류산염( PMS)/록화초기사담서람( NBT)현색체계,차조응효성상의화보통조상궤량충성상방식,건립료지기미공진렬심편비색법검측유산탈경매( LDH)적방법。재최가실험조건하,현색강도여LDH농도정선성상관。채용응효성상의검측시,선성범위위10~150 U/L,검출한(3σ)위9.44 U/L(n=18)。채용조상법획득적선성범위위15~150 U/L,검출한(3σ)위12.36 U/L(n=18)。실험표명,인혈청백단백(HSA)대현색결과구유증강작용,탐토료HSA적증색작용,병이HSA위증강시제득도공작곡선。기우지기미공진렬심편적LDH활성측정방법구유조작간단、결과직관가견、령민도고등우점,대우탈경매류적편첩검측유일정삼고개치,가망재생물의료검측영역획득응용。
A low-cost, simple, sensitive detection method of lactate dehydrogense ( LDH) was developed on paper-based microwell arrays microfluidic device. The phenazine methyl sulfate/nitrotetrazolium blue chloride ( PMS/NBT) detection system was used for LDH detection and the colorimetric results were recorded by both Gel Documentation System and a common camera. Under the optimized conditions, the colorimetric intensity showed a linear correlation to the activity of LDH in the range of 10 to 150 U/L with a limit of detection (LOD) of 9. 44 U/L (3σ) by Gel Documentation System;and the linear range was 15-150 U/L by camera with a LOD of 12. 36 U/L (3σ). Foremost, it was found that human serum albumin (HSA) had an effect on the colorimetric enhancement in this detection system. This low-cost, portable paper-based analytical platform could be suitable for the application in the point-of-care with high sensitivity and reproducibility.