海南医学
海南醫學
해남의학
HAINAN MEDICAL JOURNAL
2014年
13期
1958-1960
,共3页
乙型肝炎病毒%HBV-DNA%血清%乳汁
乙型肝炎病毒%HBV-DNA%血清%乳汁
을형간염병독%HBV-DNA%혈청%유즙
Hepatitis B virus%HBV-DNA%Serum%Milk
目的：通过检测分析乙肝病毒携带产妇血清学标志物与血清、乳汁HBV-DNA阳性率的关系，以及产妇血清与乳汁中HBV-DNA含量之间的相关性，旨在指导母乳喂养。方法选取96例乙肝病毒携带产妇，将其分为大三阳组(54例)、小三阳组(25例)、HbsAg和HbeAg均阳性组（8例）及HbsAg和HbcAb均阳性组（9例）。另选取12例乙肝两对半全阴的产妇作为对照组。ELISA法检测乙肝病毒携带产妇乙肝免疫血清学标志物，实时荧光定量PCR法分别检测产妇血清与乳汁中HBV-DNA含量，并对所有检测指标进行相关性分析。结果大三阳组产妇血清和乳汁HBV-DNA阳性率明显高于其他三组(P<0.05)。乳汁HBV-DNA在各组中检出的阳性率均小于血清HBV-DNA，但两者差异无统计学意义(P>0.05)。根据乙型肝炎血清学标志物HBeAg是否阳性将96例产妇分为HBeAg阳性组(62例)和HBeAg阴性组(34例)，血清HBeAg阳性产妇的血清和乳汁中HBV-DNA阳性率均明显高于HBeAg阴性产妇，差异具有统计学意义(P<0.01)。但一部分血清HBeAg阴性产妇血清和乳汁中HBV-DNA亦为阳性。产妇血清与乳汁中HBV-DNA含量呈正相关(r=0.891，P<0.05)。结论乙肝病毒携带产妇乳汁HBV-DNA检出的阳性率低于血清HBV-DNA，且乳汁HBV-DNA含量随血清HBV-DNA含量的升高而增大，因此定量双重检测产妇血清、乳汁中HBV-DNA来确定母婴乙肝病毒传播的风险性更为可靠，这将有利于阻断乙肝传播，确定哺乳方式，指导母乳喂养，从而降低乙肝新生儿感染率。
目的：通過檢測分析乙肝病毒攜帶產婦血清學標誌物與血清、乳汁HBV-DNA暘性率的關繫，以及產婦血清與乳汁中HBV-DNA含量之間的相關性，旨在指導母乳餵養。方法選取96例乙肝病毒攜帶產婦，將其分為大三暘組(54例)、小三暘組(25例)、HbsAg和HbeAg均暘性組（8例）及HbsAg和HbcAb均暘性組（9例）。另選取12例乙肝兩對半全陰的產婦作為對照組。ELISA法檢測乙肝病毒攜帶產婦乙肝免疫血清學標誌物，實時熒光定量PCR法分彆檢測產婦血清與乳汁中HBV-DNA含量，併對所有檢測指標進行相關性分析。結果大三暘組產婦血清和乳汁HBV-DNA暘性率明顯高于其他三組(P<0.05)。乳汁HBV-DNA在各組中檢齣的暘性率均小于血清HBV-DNA，但兩者差異無統計學意義(P>0.05)。根據乙型肝炎血清學標誌物HBeAg是否暘性將96例產婦分為HBeAg暘性組(62例)和HBeAg陰性組(34例)，血清HBeAg暘性產婦的血清和乳汁中HBV-DNA暘性率均明顯高于HBeAg陰性產婦，差異具有統計學意義(P<0.01)。但一部分血清HBeAg陰性產婦血清和乳汁中HBV-DNA亦為暘性。產婦血清與乳汁中HBV-DNA含量呈正相關(r=0.891，P<0.05)。結論乙肝病毒攜帶產婦乳汁HBV-DNA檢齣的暘性率低于血清HBV-DNA，且乳汁HBV-DNA含量隨血清HBV-DNA含量的升高而增大，因此定量雙重檢測產婦血清、乳汁中HBV-DNA來確定母嬰乙肝病毒傳播的風險性更為可靠，這將有利于阻斷乙肝傳播，確定哺乳方式，指導母乳餵養，從而降低乙肝新生兒感染率。
목적：통과검측분석을간병독휴대산부혈청학표지물여혈청、유즙HBV-DNA양성솔적관계，이급산부혈청여유즙중HBV-DNA함량지간적상관성，지재지도모유위양。방법선취96례을간병독휴대산부，장기분위대삼양조(54례)、소삼양조(25례)、HbsAg화HbeAg균양성조（8례）급HbsAg화HbcAb균양성조（9례）。령선취12례을간량대반전음적산부작위대조조。ELISA법검측을간병독휴대산부을간면역혈청학표지물，실시형광정량PCR법분별검측산부혈청여유즙중HBV-DNA함량，병대소유검측지표진행상관성분석。결과대삼양조산부혈청화유즙HBV-DNA양성솔명현고우기타삼조(P<0.05)。유즙HBV-DNA재각조중검출적양성솔균소우혈청HBV-DNA，단량자차이무통계학의의(P>0.05)。근거을형간염혈청학표지물HBeAg시부양성장96례산부분위HBeAg양성조(62례)화HBeAg음성조(34례)，혈청HBeAg양성산부적혈청화유즙중HBV-DNA양성솔균명현고우HBeAg음성산부，차이구유통계학의의(P<0.01)。단일부분혈청HBeAg음성산부혈청화유즙중HBV-DNA역위양성。산부혈청여유즙중HBV-DNA함량정정상관(r=0.891，P<0.05)。결론을간병독휴대산부유즙HBV-DNA검출적양성솔저우혈청HBV-DNA，차유즙HBV-DNA함량수혈청HBV-DNA함량적승고이증대，인차정량쌍중검측산부혈청、유즙중HBV-DNA래학정모영을간병독전파적풍험성경위가고，저장유리우조단을간전파，학정포유방식，지도모유위양，종이강저을간신생인감염솔。
Objective Through analysis of the relationship between the hepatitis B serum markers in HBV-infectious pregnant women and the HBV-DNA positive rate in serum and milk, and the correlation serum and milk HBV-DNA in HBV-infectious pregnant woman, we aimed to explore some instructions on breast-feeding. Methods Ninety-six HBV-infectious pregnant women were divided into four groups, including large three-positive group, small three-positive group, HbsAg and HbeAg positive group, and HbsAg and HbcAb positive group. Another 12 women with hepatitis B two pairs of semi-five indicators all negative were chosen as control group. The hepatitis B virus immune markers in serum were detected by ELISA while HBV-DNA level in serum and milk was determined by real time RT-PCR, and the correlation between the detecting results were analyzed. Results The positive rates of HBV-DNA in serum and milk of large three positive group were significantly higher than those in the other three groups (P<0.05). Among all groups, the positive rates of HBV-DNA in milk were less than those in serum with no sig-nificant difference (P>0.05). The HBV-DNA positive rates in serum and milk in HBeAg positive groups were obvious-ly higher than that in HBeAg-negative group (P<0.01). However, HBV-DNA in serum and milk were also detected in part of the HBeAg-negative pregnancy women. The HBV-DNA content in serum had positive relation with HBV-DNA content in milk (r=0.891, P<0.05). Conclusion In HBV- infectious pregnant women, it is found that the HBV-DNA positive rate in milk was less than that in serum, and the content of HBV-DNA in milk was increased along with that increased in serum. Therefore, it is more reliable to determine the risk of hepatitis B virus transmission from mother to infant by quantitative measurement of HBV-DNA in serum and milk. It is helpful in interrupting HBV trans-mission, deciding the mode of breast-feeding, and guiding breast-feeding, so as to decrease the infectious rate of baby.
