现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2014年
15期
89-91
,共3页
台湾过山香%药用植物%组织培养%快繁%基质
檯灣過山香%藥用植物%組織培養%快繁%基質
태만과산향%약용식물%조직배양%쾌번%기질
Clausena excavata Burm. f.%medicinal plant%tissue culture%rapid propagation%stroma
以当年生台湾过山香带腋芽半木质化茎段作为外植体，开展组织培养试验研究，建立组培快繁体系。结果表明：最佳外植体诱导培养基为1/2MS+6-BA 1.0 mg/L+NAA 0.1 mg/L，诱导率可达89.7%；最佳增殖培养基为DCR+6-BA 0.8 mg/L+NAA 0.1 mg/L，增殖率达5.24倍，继代周期为35 d，不定芽生长状况好；最佳生根培养基为1/2MS+IBA 0.5 mg/L+ABT 1生根粉0.4 mg/L，生根率达95.26%；以泥炭土：黄心土：珍珠岩：钙镁磷肥(3.0：2.0：1.0：0.2)，移栽成活率达93.7%，且7 d缓苗，茎秆木质化迅速，茎叶浓绿，长势好。
以噹年生檯灣過山香帶腋芽半木質化莖段作為外植體，開展組織培養試驗研究，建立組培快繁體繫。結果錶明：最佳外植體誘導培養基為1/2MS+6-BA 1.0 mg/L+NAA 0.1 mg/L，誘導率可達89.7%；最佳增殖培養基為DCR+6-BA 0.8 mg/L+NAA 0.1 mg/L，增殖率達5.24倍，繼代週期為35 d，不定芽生長狀況好；最佳生根培養基為1/2MS+IBA 0.5 mg/L+ABT 1生根粉0.4 mg/L，生根率達95.26%；以泥炭土：黃心土：珍珠巖：鈣鎂燐肥(3.0：2.0：1.0：0.2)，移栽成活率達93.7%，且7 d緩苗，莖稈木質化迅速，莖葉濃綠，長勢好。
이당년생태만과산향대액아반목질화경단작위외식체，개전조직배양시험연구，건립조배쾌번체계。결과표명：최가외식체유도배양기위1/2MS+6-BA 1.0 mg/L+NAA 0.1 mg/L，유도솔가체89.7%；최가증식배양기위DCR+6-BA 0.8 mg/L+NAA 0.1 mg/L，증식솔체5.24배，계대주기위35 d，불정아생장상황호；최가생근배양기위1/2MS+IBA 0.5 mg/L+ABT 1생근분0.4 mg/L，생근솔체95.26%；이니탄토：황심토：진주암：개미린비(3.0：2.0：1.0：0.2)，이재성활솔체93.7%，차7 d완묘，경간목질화신속，경협농록，장세호。
Stems with axillary buds from Clausena excavata Burm. f. were used as explants for tissue culture and tissue culture was establishment. The results showed that the suitable medium of explants inducing was 1/2MS supplemented with 6-BA 1.0 mg/L and NAA 0.1 mg/L,and the inducing rate was 89.7%.The DCR supplemented with 6-BA 0.8 mg/L and NAA 0.1 mg/L was the optimized proliferating medium with multiplication coefficient being 5.24 and the growth cycle being 35 d,and the shoots grew well. The best rooting medium was 1/2MS supplemented with IBA 0.5 mg/L and ABT 1 rooting power 0.4 mg/L,and the rooting rate was 95.26%.The plantlets were transplanted in mixture media with peat,yellow soil and perlite calcium magnesium phosphate(3.0:2.0:1.0:0.2),and the survival rate was 93.7%.And 7 d later,the seedling was survival,stem was quickly lignification,leaf was deeper green and growing well.
