CAJ | 학술논문

目的：研究基底细胞乳头状瘤（basal cell papilloma，BCP）患者曝光部位皮损、非曝光部位皮损以及正常皮肤组织中MMP-1和PTEN蛋白的表达，探讨BCP的发病与皮肤光老化之间的关系。方法：选取曝光部位皮损50例，非曝光部位皮损50例，正常皮肤30例。采用免疫组织化学Elivison法检测各组MMP-1和PTEN蛋白的表达情况，比较各组间差异。选取曝光部位皮损90例，分为3组，分别为外用遮光剂二氧化钛霜组、安慰剂组和未用药组，用药3个月后比较3组皮损MMP-1的表达。结果：曝光部位皮损MMP-1的表达评分较正常对照组显著升高（P<0.01），非曝光部位皮损MMP-1的表达评分与对照组无显著性差异（P>0.05）。曝光部位皮损和非曝光部位皮损PTEN蛋白的表达评分均较正常对照组显著降低（P<0.01）。3个月后，外用二氧化钛霜组MMP-1的表达评分较未用药组明显降低（P<0.05）。结论：MMP-1在曝光部位的BCP中呈现高表达。PTEN蛋白在曝光部位和非曝光部位的BCP中呈现低表达。紫外线照射导致的皮肤光老化可能是曝光部位BCP发生的一个诱发因素。
목적：연구기저세포유두상류（basal cell papilloma，BCP）환자폭광부위피손、비폭광부위피손이급정상피부조직중MMP-1화PTEN단백적표체，탐토BCP적발병여피부광노화지간적관계。방법：선취폭광부위피손50례，비폭광부위피손50례，정상피부30례。채용면역조직화학Elivison법검측각조MMP-1화PTEN단백적표체정황，비교각조간차이。선취폭광부위피손90례，분위3조，분별위외용차광제이양화태상조、안위제조화미용약조，용약3개월후비교3조피손MMP-1적표체。결과：폭광부위피손MMP-1적표체평분교정상대조조현저승고（P<0.01），비폭광부위피손MMP-1적표체평분여대조조무현저성차이（P>0.05）。폭광부위피손화비폭광부위피손PTEN단백적표체평분균교정상대조조현저강저（P<0.01）。3개월후，외용이양화태상조MMP-1적표체평분교미용약조명현강저（P<0.05）。결론：MMP-1재폭광부위적BCP중정현고표체。PTEN단백재폭광부위화비폭광부위적BCP중정현저표체。자외선조사도치적피부광노화가능시폭광부위BCP발생적일개유발인소。
Objective:To investigate the expression of MMP-1 and PTEN protein in basal cell papilloma (BCP), as well as their correlation with skin photoaging. Methods:Immunohistochemistry technique via Elivison method was employed to measure the expres-sion of MMP-1 and PTEN protein in lesions from 50 cases of BCP on exposed areas, 50 cases on non-exposed areas, and 30 normal controls. We compared the differences among the three groups and analyzed the result. A total of 90 BCP cases on exposed areas were randomly divided into three groups. Titanium dioxide cream and placebo were respectively applied in the trial groups twice daily for 12 weeks, whereas the control group was non-administered. After 12 weeks, the MMP-1 in the lesions of the three groups was measured and compared. Results:The expression scores of MMP-1 on exposed areas were significantly higher than those in the control group (P<0.01). No significant difference was found between non-exposed areas and the control group (P>0.05). The expression scores of PTEN protein on exposed areas and on non-exposed areas were significantly lower than that in control group (P<0.01). The expression scores of MMP-1 in the group that used titanium dioxide were evidently lower than those in control group after 12 weeks (P<0.05). Conclu-sion:MMP-1 is overexpressed in BCP on exposed areas. PTEN protein is underexpressed in BCP of exposed areas and non-exposed ar-eas. Skin photoaging is a possible cause of BCP on exposed areas.