食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
16期
101-103,104
,共4页
放线菌%纤维素酶%响应面
放線菌%纖維素酶%響應麵
방선균%섬유소매%향응면
Actinomycetees%cellulase%response surface
采用紫外与60Co-γ射线诱变获得的高产纤维素酶菌株系为研究对象，研究产纤维素酶的发酵条件，结果表明：通过单因素实验，确定复合碳源，蛋白胨，KH2PO43个因素的取值范围。利用SAS9.1软件对碳源、氮源以及KH2PO4进行响应面分析。结果表明：X1（氮源）=4.05 g，X2（复合碳源）=3.69 g， X3（KH2PO4）=4.68%，CMCase最大酶活为94.8688 IU/mL。
採用紫外與60Co-γ射線誘變穫得的高產纖維素酶菌株繫為研究對象，研究產纖維素酶的髮酵條件，結果錶明：通過單因素實驗，確定複閤碳源，蛋白胨，KH2PO43箇因素的取值範圍。利用SAS9.1軟件對碳源、氮源以及KH2PO4進行響應麵分析。結果錶明：X1（氮源）=4.05 g，X2（複閤碳源）=3.69 g， X3（KH2PO4）=4.68%，CMCase最大酶活為94.8688 IU/mL。
채용자외여60Co-γ사선유변획득적고산섬유소매균주계위연구대상，연구산섬유소매적발효조건，결과표명：통과단인소실험，학정복합탄원，단백동，KH2PO43개인소적취치범위。이용SAS9.1연건대탄원、담원이급KH2PO4진행향응면분석。결과표명：X1（담원）=4.05 g，X2（복합탄원）=3.69 g， X3（KH2PO4）=4.68%，CMCase최대매활위94.8688 IU/mL。
Study fermentation conditions, an excellent cellulase-producing strain, which was screened by UV and 60Co-γray was researched. The ranges of mixture carbon,peptone, KH2PO4 were confirmed through single factor experiment.Carbon and Nitrogen sources and KH2PO4 were analysised in response surface by using of SAS9.1 software. The results showed that mixture carbon 3.69 g, Nitrogen 4.05 g, KH2PO4 4.68 %,the max cellulase activity was 94.868 8 IU/mL.
