CAJ | 학술논문

建立了超高效液相色谱检测原料乳中双氰胺的分析方法。采用甲醇提取样品中的双氰胺，HILICS色谱柱为分析柱，流动相为乙腈-10 mmol/L乙酸铵（90∶10，体积比），流速0.4 mL/min，检测波长230 nm。双氰胺在0.006 mg/L～10.0 mg/L范围内具有良好的线性关系，标准曲线方程为y=90.336x+0.9946，R2=0.9999。方法检出限为0.05 mg/kg，不同添加水平平均回收率在87.0%～91.6%之间，变异系数＜3.84%（n=6）。此方法操作简便，可在15 min内完成快速检测，适用于原料乳中双氰胺残留的快速筛查。
건립료초고효액상색보검측원료유중쌍청알적분석방법。채용갑순제취양품중적쌍청알，HILICS색보주위분석주，류동상위을정-10 mmol/L을산안（90∶10，체적비），류속0.4 mL/min，검측파장230 nm。쌍청알재0.006 mg/L～10.0 mg/L범위내구유량호적선성관계，표준곡선방정위y=90.336x+0.9946，R2=0.9999。방법검출한위0.05 mg/kg，불동첨가수평평균회수솔재87.0%～91.6%지간，변이계수＜3.84%（n=6）。차방법조작간편，가재15 min내완성쾌속검측，괄용우원료유중쌍청알잔류적쾌속사사。
An ultra high performance liquid chromatography method was developed for determination of dicyandiamide in saw milk. Dicyandiamide was extracted by methanol, the sample was isolated on a HILIC column,using a mobile phase made up of acetonitrile and 10mmol/L ammonium acetate (90∶10, V/V) at a flow rate of 0.4 mL/min, and detected at 230 nm. Dicyandiamide was well separated with a good linear relationship in the range of 0.006 mg/L-10.0 mg/L,caffeine standard curve equation:y=90.336x+ 0.994 6,R2=0.999 9.The detection limit was 0.05 mg/kg, recovery rate was in the range of 87.0%-91.6%at several spiked levels, and the coefficient of variance of method was less than 3.84%(n=6).This method is simple, the whole determination process was completed within 15 min, making it applicable for analysis in dicyandiamide monitoring programmes in saw milk.