CAJ | 학술논문

通过分析20条不同柑橘属植物的trnL基因序列，设计柑桔属植物特异性扩增引物。通过对苹果、梨、葡萄等多种水果成分的特异性筛选，建立检测柑桔属植物成分的SYB Green实时荧光PCR检测方法，该方法对柑桔DNA的检测低限为0.1 pg/μL。用该方法检测10种橙汁及其饮料商品，结果表明，该方法能够检测到橙汁中的柑桔属植物成分。该方法可用于果汁或食品中柑桔属植物成分的鉴别。
통과분석20조불동감귤속식물적trnL기인서렬，설계감길속식물특이성확증인물。통과대평과、리、포도등다충수과성분적특이성사선，건립검측감길속식물성분적SYB Green실시형광PCR검측방법，해방법대감길DNA적검측저한위0.1 pg/μL。용해방법검측10충등즙급기음료상품，결과표명，해방법능구검측도등즙중적감길속식물성분。해방법가용우과즙혹식품중감길속식물성분적감별。
A real-time fluorescence polymerase chain reaction method using the fluorescence dye SYBR Green and aimed at citrus trnL gene sequence was established for detection of citrus DNA in fruit juice. The citrus-specific primers were designed according to the sequence alignment result between 20 different citrus trnL gene sequences. The citrus component could be detected by the citrus-specific primers with a limit of detection of 0.1 pg/μL DNA. The following detection of 10 fruit juice and beverage samples showed that this method was suitable to identify the citrus component in fruit juice beverage and other relative food and can provide a technique base for research of fruit juice adulteration detection.