CAJ | 학술논문

采用响应面法对蜂胶总黄酮提取工艺进行优化。以蜂胶原胶为原料，基于单因素实验，以乙醇体积分数、料液比、提取时间和提取次数为相应因素，蜂胶总黄酮提取率为响应值，根据中心组合Box-Behnken的中心组合试验设计原理，采用四因素三水平响应面分析法进行响应面试验，并对各个因素的显著性和交互作用进行分析。结果表明，最优工艺条件为提取次数2次、料液比1∶45（g/mL）、提取时间30 min、乙醇浓度90%，在此条件下，总黄酮的提取率为17.07%。
채용향응면법대봉효총황동제취공예진행우화。이봉효원효위원료，기우단인소실험，이을순체적분수、료액비、제취시간화제취차수위상응인소，봉효총황동제취솔위향응치，근거중심조합Box-Behnken적중심조합시험설계원리，채용사인소삼수평향응면분석법진행향응면시험，병대각개인소적현저성화교호작용진행분석。결과표명，최우공예조건위제취차수2차、료액비1∶45（g/mL）、제취시간30 min、을순농도90%，재차조건하，총황동적제취솔위17.07%。
Propolis flavonoids were extracted by ethanol, and the extraction process was optimized by response surface analysis. On the basis of one-factor-at-a-time experiments, the optimal extraction conditions for maximizing propolis flavonoids were determined by using a 4-variable, 3-level Box-Behnken experimental design combined with response surface analysis as follows:the extraction time was 2;the liquid-material ratio was 1∶45(g/mL);the extraction time was 30 min;the ethanol concentration was 90%(volume ratio), resulting in anpropolis flavonoids yield of 17.07%.