CAJ | 학술논문

目的探讨评价骨髓单个核细胞(bone marrow mononuclear cells,BMMNCs)移植对大脑中动脉栓塞小鼠脑白质损伤的疗效及梗死周围血管再生的影响.方法线栓法将成年雄性小鼠制成大脑中动脉栓塞模型,模型成功后按随机表法随机分成假手术组,MCAO组,PBS组及BMMNCs移植组(BMMNCs组).梯度密度离心法提取分离BMMNCs,模型成功后24 h经尾静脉移植入0.5 ml含1×106个BMMNCs细胞悬液,PBS组给予等体积的PBS溶液,神经功能学评分(mNSS)评价小鼠神经功能,luxol fast blue(LFB)染色观察梗死周围脑白质损伤情况,免疫荧光染色观察梗死周围血管再生情况.结果除假手术组外,其他各组均有不同程度的神经功能缺损.梗死后56 d,BMMNCs组LFB着色减少百分比[(24.06±5.48)％]明显低于MCAO组[(62.22±5.38)％]及PBS组[(61.22±5.48)％],BMMNCs组血管密度[(249.8±11.8)个/mm2]明显高于MCAO组[(119.1 ±11.2)个/mm2]及PBS组[(114.3±12.1)个/mm2],差异有统计学意义(P＜0.05),MCAO组与PBS组相比,LFB着色减少百分比和血管密度差异无统计学意义(P＞0.05).结论 BMMNCs移植可促进血管新生,增加梗死周围区血管密度,减轻脑白质损伤程度,显著改善脑梗死小鼠神经功能.
목적 탐토평개골수단개핵세포(bone marrow mononuclear cells,BMMNCs)이식대대뇌중동맥전새소서뇌백질손상적료효급경사주위혈관재생적영향.방법 선전법장성년웅성소서제성대뇌중동맥전새모형,모형성공후안수궤표법수궤분성가수술조,MCAO조,PBS조급BMMNCs이식조(BMMNCs조).제도밀도리심법제취분리BMMNCs,모형성공후24 h경미정맥이식입0.5 ml함1×106개BMMNCs세포현액,PBS조급여등체적적PBS용액,신경공능학평분(mNSS)평개소서신경공능,luxol fast blue(LFB)염색관찰경사주위뇌백질손상정황,면역형광염색관찰경사주위혈관재생정황.결과 제가수술조외,기타각조균유불동정도적신경공능결손.경사후56 d,BMMNCs조LFB착색감소백분비[(24.06±5.48)％]명현저우MCAO조[(62.22±5.38)％]급PBS조[(61.22±5.48)％],BMMNCs조혈관밀도[(249.8±11.8)개/mm2]명현고우MCAO조[(119.1 ±11.2)개/mm2]급PBS조[(114.3±12.1)개/mm2],차이유통계학의의(P＜0.05),MCAO조여PBS조상비,LFB착색감소백분비화혈관밀도차이무통계학의의(P＞0.05).결론 BMMNCs이식가촉진혈관신생,증가경사주위구혈관밀도,감경뇌백질손상정도,현저개선뇌경사소서신경공능.
Objective To evaluate the effect of transplanting bone marrow mononuclear cells (BMMNCs)for white matter damage and angiogenesis in cerebral infarction mice.Methods The male adult mice were randomly divided into four groups:Sham operated group,MCAO group,PBS group and BMMNCs transplantation group (BMMNCs group).The MCAO mice in last three groups were induced by occlusion of the right middle cerebral artery using an intra luminal filament technique.The successful model was injected with 0.5 ml cell suspension containing 1 × 106 BMMNCs via tail vein after 24h,and the PBS group were injected with the same volume of PBS.The effect of bone marrow mononuclear cells transplantation in mice were observed by recording the neurological scores,using the luxol fast blue (LFB) staining to assess the severity of white matter damage in peri-infarction zone,and the vWF immunofluorescence to investigate the vessel density in peri-infarction zone.Results Except for the sham operated group,the other groups suffered from different level of neurological deficits.After the 56 days of stroke,the reduced percent of white matter marked by LFB in BMMNCs group ((24.06 ± 5.48) ％) was significantly lower(P ＜ 0.05) than that in MCAO group ((62.22 ± 5.38) ％) and that in PBS group ((61.22 ±5.48) ％),and the density of vessel in peri-infarction zone in BMMNCs group (249.8 ± 11.8/mm2) was significantly lower(P ＜ 0.05) than that in MCAO group (119.1 ± 11.2/mm2) and that in PBS group (114.3 ± 12.1/mm2).No difference was showed between the MCAO group and PBS group(P＞ 0.05).Conclusion Intravenously transplanted BMMNCs can promote the angiogenesis,increase the density of vessel in peri-infarction area,alleviate the white matter damage and stimulate the recovery of neurological function.