CAJ | 학술논문

目的观察丙戊酸钠(VPA)孤独症模型鼠海马脑源性神经营养因子(BDNF)阳性神经元表达及锥体细胞形态学改变.方法按Schneider方法制作VPA孤独症动物模型,采用免疫组化和图像分析技术检测模型鼠海马CA1区BDNF阳性神经元表达水平及海马CA1区锥体细胞形态学的改变.结果孤独症模型组与正常对照组比较,海马CA1区锥体细胞BDNF阳性神经元表达水平增强,孤独症模型组与正常对照组阳性细胞数分别为(5.00±1.60)/视野和(3.00±1.04)/视野,差异有统计学意义(t=3.63,P=0.0015)；海马CA1区锥体细胞形态学显示,孤独症模型鼠海马CA1区锥体神经元发生凋亡增加.结论孤独症的发病可能与海马CA1区锥体细胞BDNF表达水平以及锥体神经细胞的凋亡有关.
목적 관찰병무산납(VPA)고독증모형서해마뇌원성신경영양인자(BDNF)양성신경원표체급추체세포형태학개변.방법 안Schneider방법제작VPA고독증동물모형,채용면역조화화도상분석기술검측모형서해마CA1구BDNF양성신경원표체수평급해마CA1구추체세포형태학적개변.결과 고독증모형조여정상대조조비교,해마CA1구추체세포BDNF양성신경원표체수평증강,고독증모형조여정상대조조양성세포수분별위(5.00±1.60)/시야화(3.00±1.04)/시야,차이유통계학의의(t=3.63,P=0.0015)；해마CA1구추체세포형태학현시,고독증모형서해마CA1구추체신경원발생조망증가.결론 고독증적발병가능여해마CA1구추체세포BDNF표체수평이급추체신경세포적조망유관.
Objective To explore the pathogenesis of autism by observation of changes of brain-derived neurotrophic factor(BDNF) positive neurons and the morphology of pyramidal cells in hippocampal CA1 region,and provide theoretical evidence for the therapeutic schedule.Methods Animal model of autism was obtained by Schneider method.Using the immunohistochemistry methods and image analysis,the number of BDNF positive neurons was examined in hippocampal CA1 region of the autism model rats and the normal rats,and the changes of pyramidal cell were observed in hippocampal CA1 region after HE staining.Results The numbers of BDNF positive neurons in the hippocampal CA1 region of the autism model rats were more than those of the normal rats (5.00 ±1.60 vs 3.00 ± 1.04,t =3.63,P =0.0015).The morphology of pyramidal cells showed that the pyramidal cells of the autism model rats in hippocampal CA1 region had apoptosis.Conclusion The occurrence of autism may be related to the changes of BDNF and the morphology of pyramidal cells in hippocampal CA1 region.