广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2014年
16期
75-77,166
,共4页
CdTe/CdS QDs%木犀草素%人血清白蛋白%结合力%荧光猝灭
CdTe/CdS QDs%木犀草素%人血清白蛋白%結閤力%熒光猝滅
CdTe/CdS QDs%목서초소%인혈청백단백%결합력%형광졸멸
CdTe/CdS QDs%luteolin%human serum albumin%affinities%fluorescence quenching
用还原型谷胱甘肽( GSH)作硫源和稳定剂在水相中合成了生物相容性好、量子产率高的核壳型CdTe/CdS半导体量子点;同时模拟人体内生理环境,研究了CdTe/CdS量子点( QDs)对木犀草素与人血清白蛋白( HSA)相互作用的影响。结果表明, CdTe/CdS QDs可有规律地猝灭HSA荧光;在CdTe/CdS QDs存在条件下,木犀草素可使HSA荧光最大发射波长从341 nm红移到347 nm,红移程度明显大于无QDs存在时,同时木犀草素与HSA的表观结合常数和结合位点数均有增加,即CdTe/CdS QDs使木犀草素与HSA的结合力增强。
用還原型穀胱甘肽( GSH)作硫源和穩定劑在水相中閤成瞭生物相容性好、量子產率高的覈殼型CdTe/CdS半導體量子點;同時模擬人體內生理環境,研究瞭CdTe/CdS量子點( QDs)對木犀草素與人血清白蛋白( HSA)相互作用的影響。結果錶明, CdTe/CdS QDs可有規律地猝滅HSA熒光;在CdTe/CdS QDs存在條件下,木犀草素可使HSA熒光最大髮射波長從341 nm紅移到347 nm,紅移程度明顯大于無QDs存在時,同時木犀草素與HSA的錶觀結閤常數和結閤位點數均有增加,即CdTe/CdS QDs使木犀草素與HSA的結閤力增彊。
용환원형곡광감태( GSH)작류원화은정제재수상중합성료생물상용성호、양자산솔고적핵각형CdTe/CdS반도체양자점;동시모의인체내생리배경,연구료CdTe/CdS양자점( QDs)대목서초소여인혈청백단백( HSA)상호작용적영향。결과표명, CdTe/CdS QDs가유규률지졸멸HSA형광;재CdTe/CdS QDs존재조건하,목서초소가사HSA형광최대발사파장종341 nm홍이도347 nm,홍이정도명현대우무QDs존재시,동시목서초소여HSA적표관결합상수화결합위점수균유증가,즉CdTe/CdS QDs사목서초소여HSA적결합력증강。
Biocompatible and high luminescence CdTe/CdS core -shell quantum dots ( QDs ) were synthesized in aqueous solution using glutathione ( GSH) as sulfur source and stabilizer.Further, luteolin was studied for the affinities for human serum albumin ( HSA) in the presence and absence of CdTe/CdS QDs, respectively.Results revealed that the fluorescence intensities of HSA decreased remarkably with increasing concentration of QDs.Luteolin resulted in obvious red-shift of the maximum emission of HSA from 341 nm to 347 nm in the presence of QDs and the extent of red -shift induced by luteolin was much bigger than that in the absence of QDs.The binding constants and the number of binding sites per HSA molecule ( n) both increased.Thus, the binding affinities of luteolin for HSA increased in the presence of CdTe/CdS QDs.