化学与生物工程
化學與生物工程
화학여생물공정
CHEMISTRY & BIOENGINEERING
2014年
11期
31-33
,共3页
冷凤%孔鹏%谢新宇%邓佩佩%宋盼%李梅%李敏%孟雅娟
冷鳳%孔鵬%謝新宇%鄧珮珮%宋盼%李梅%李敏%孟雅娟
랭봉%공붕%사신우%산패패%송반%리매%리민%맹아연
棘白霉素 B 母核%聚合物纳米微球%分离纯化
棘白黴素 B 母覈%聚閤物納米微毬%分離純化
극백매소 B 모핵%취합물납미미구%분리순화
echinocandin B nucleus%polymer nanoparticles%separation and purification
以聚合物纳米微球为层析介质，采用 HPLC 检测方法，开发高纯度棘白霉素 B 母核的分离纯化工艺，并对层析条件进行优化。结果表明，当选择 Uni PS 30型聚合物纳米微球为层析介质、上样量为1.2 g·（100 g 介质）－1、以体积分数为3％的乙醇进行洗脱、流速为12 mL·min－1时，得到的棘白霉素 B 母核纯度大于98％。建立了高纯度棘白霉素 B 母核的分离纯化工艺，流程简单、可行，为该产品产业化开发提供了一定的理论基础。
以聚閤物納米微毬為層析介質，採用 HPLC 檢測方法，開髮高純度棘白黴素 B 母覈的分離純化工藝，併對層析條件進行優化。結果錶明，噹選擇 Uni PS 30型聚閤物納米微毬為層析介質、上樣量為1.2 g·（100 g 介質）－1、以體積分數為3％的乙醇進行洗脫、流速為12 mL·min－1時，得到的棘白黴素 B 母覈純度大于98％。建立瞭高純度棘白黴素 B 母覈的分離純化工藝，流程簡單、可行，為該產品產業化開髮提供瞭一定的理論基礎。
이취합물납미미구위층석개질，채용 HPLC 검측방법，개발고순도극백매소 B 모핵적분리순화공예，병대층석조건진행우화。결과표명，당선택 Uni PS 30형취합물납미미구위층석개질、상양량위1.2 g·（100 g 개질）－1、이체적분수위3％적을순진행세탈、류속위12 mL·min－1시，득도적극백매소 B 모핵순도대우98％。건립료고순도극백매소 B 모핵적분리순화공예，류정간단、가행，위해산품산업화개발제공료일정적이론기출。
Using polymer nanoparticles as the chromatography medium,a method for separation and purifi-cation of high-purity echinocandin B nucleus was developed.The purity was detected by HPLC,the chromato-graphic conditions were optimized.Results showed that,when selecting polymer nanoparticles Uni PS 30 as the chromatography medium,loading capacity was 1.2 g·(100 g medium)-1 ,volume fraction of ethanol was 3%, and the flow rate was 12 mL·min-1 ,echinocandin B nucleus with the purity of 98% were obtained.The meth-od for separation and purification of echinocandin B nucleus was established.It was simple and feasible,which provided a theoretical basis for the industrialization of echinocandin B nucleus.
