CAJ | 학술논문

为掌握从谷氨酸发酵液提取生物基尼龙聚丁内酰胺单体γ-氨基丁酸（ GABA）的技术，本文研究了膜过滤联合离子交换吸附和洗脱的工艺，并且使用活性炭进行脱色。膜过滤过程中初始大跨膜压差为0．85 bar，过滤温度控制在38℃；离子交换过程采用QY-021-a强酸型阳离子交换树脂，常温下吸附-洗脱的操作方法；经旋转蒸发浓缩后，添加15％（碳对固形物含量之比）活性炭，温度为65℃，搅拌40 min脱色。结果表明：平均膜通量为128 L/m2·h，浓缩倍数为33．3倍。经取样检测膜透过液样品，浓缩液样品计算GABA收率为97．7％，微滤膜以及膜表面污染物上无GABA截留和吸附截留；离子交换经5 BV洗脱后计算收率，吸附-洗脱过程的收率为92．8％；使用活性碳B的脱色率为94．2％，GABA收率为99．2％。该工艺的总收率为85％，该工艺具有工业化应用前景，并且可以通过优化离子交换工艺和设备进一步提高收率。
위장악종곡안산발효액제취생물기니룡취정내선알단체γ-안기정산（ GABA）적기술，본문연구료막과려연합리자교환흡부화세탈적공예，병차사용활성탄진행탈색。막과려과정중초시대과막압차위0．85 bar，과려온도공제재38℃；리자교환과정채용QY-021-a강산형양리자교환수지，상온하흡부-세탈적조작방법；경선전증발농축후，첨가15％（탄대고형물함량지비）활성탄，온도위65℃，교반40 min탈색。결과표명：평균막통량위128 L/m2·h，농축배수위33．3배。경취양검측막투과액양품，농축액양품계산GABA수솔위97．7％，미려막이급막표면오염물상무GABA절류화흡부절류；리자교환경5 BV세탈후계산수솔，흡부-세탈과정적수솔위92．8％；사용활성탄B적탈색솔위94．2％，GABA수솔위99．2％。해공예적총수솔위85％，해공예구유공업화응용전경，병차가이통과우화리자교환공예화설비진일보제고수솔。
In order to explore the extraction technology of GABA from fermentation broth with glutamic acid as substrate, membrane filtration associated with ion-exchange adsorption and desorption process were studied in this paper, and followed with decolorization by using active carbon. The initial trans-membrane pressure is 0. 85 bar during the filtration process, and the temperature was controlled at 38℃. With QY-021-a strong acid cation exchange resin as packing in ion-exchange column, adsorption-desorption process was exerted at room temperature. After concentrated by rotary evaporation, decolorization was performed using 15% ( carbon/solids in the solution ) activate carbon, with temperature fixed at 65℃, stirring for 40 min. Results showed that the average flux was 128 L/m2·h, and concentration ratio was 33.3. Samplings to detect membrane permeate showed that the yield of GABA was 97.7%, which indicated that there was no GABA retention of interception and adsorption on microfiltration membrane surface and fouling layer. After collecting 5 BV permeate to calculate the yield of ion-exchange process, results showed that the yield was 92.8%, decolorization ratio was 94.2% by using activate carbon B, and the GABA yield was 99?2%. The total yield of the whole process was up to 85%, which indicated that this process has the prospects for industrial application, and further improvements of yield could be achieved by optimized ion exchange processes and equipment.